Interaction between non-structural protein Pns10 of rice dwarf virus and cytoplasmic actin of leafhoppers is correlated with insect vector specificity
Interaction between non-structural protein Pns10 of rice dwarf virus and cytoplasmic actin of leafhoppers is correlated with insect vector specificity
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水稻矮缩病毒非结构蛋白Pns10与叶蝉细胞质肌动蛋白之间的相互作用与昆虫载体特异性相关
DOI：
10.1099/jgv.0.000022
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发表时间：
2015-04-01
影响因子：
3.8
通讯作者：
Wei, Taiyun
中科院分区：
文献类型：
--
作者：
Chen, Qian;Wang, Haitao;Wei, Taiyun
许多昆虫传播的病原体是由特定的昆虫物种传播的，而不是由其他昆虫物种传播的，即使这些昆虫物种是密切相关的。这种严格的病原体-昆虫特异性的分子机制知之甚少。水稻矮缩病毒（RDV）是一种植物呼肠孤病毒，主要由叶蝉黑尾叶蝉（Nephotettix cincticeps）传播，但由叶蝉Recilia dorsalis传播无效。在此，我们证明了由RDV的病毒非结构蛋白Pnsl 0组成的含病毒小管与N.而与R.背肌此外，RDV的Pnsl 0还能与N. cincticeps，但与R.这表明PnslO与昆虫细胞质肌动蛋白的相互作用与叶蝉对RDV的传播性一致。这些结果表明，RDV的Pnsl 0与昆虫细胞质肌动蛋白的相互作用可能决定了病原-载体特异性。
Many insect-transmissible pathogens are transmitted by specific, insect species and not by others, even if the insect species are closely related. The molecular mechanisms underlying such strict pathogen-insect specificity are poorly understood. Rice dwarf virus (RDV), a plant reovirus, is transmitted mainly by the leafhopper species Nephotettix cincticeps but is transmitted ineffectively by the leafhopper Recilia dorsalis. Here, we demonstrated that virus-containing tubules composed of viral non-structural protein Pnsl 0 of RDV associated with the intestinal microvilli of N. cincticeps but not with those of R. dorsalis. Furthermore, Pnsl 0 of RDV specifically interacted with cytoplasmic actin, the main component of microvilli of N. cincticeps, but not with that of R. dorsalis, suggesting that the interaction of Pnsl 0 with insect cytoplasmic actin is consistent with the transmissibility of RDV by leafhoppers. All these results suggested that the interaction of Pnsl 0 of RDV with insect cytoplasmic actin may determine pathogen-vector specificity.