VP1 sequencing protocol for foot and mounth disease virus molecular epidemilogy
VP1 sequencing protocol for foot and mounth disease virus molecular epidemilogy
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DOI：
10.20506/rst.35.3.2565
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发表时间：
2016-12-01
影响因子：
0.6
通讯作者：
King, D. P.
中科院分区：
文献类型：
--
作者：
Knowles, N. J.;Wadsworth, J.;King, D. P.
口蹄疫病毒（FMDV）野外株的核苷酸序列有助于我们了解在世界不同地区传播的病毒谱系的分布和进化。本文概述了一种实用的逆转录聚合酶链反应（RT-PCR）和测序策略，可用于产生编码口蹄疫病毒VP 1（1D）区的RNA序列。该方案包含一组PCR和测序引物，可以选择这些引物来扩增代表所有七种FMDV血清型的遗传多样性分离株。还描述了一系列序列，包括所有提出的FMDV拓扑型的原型序列，以提供系统发育分析的框架。本文提供的技术细节和原型序列可以被FMD参考实验室和其他机构用于协调FMDV的分子流行病学。
Nucleotide sequences of field strains of foot and mouth disease virus (FMDV) contribute to our understanding of the distribution and evolution of viral lineages that circulate in different regions of the world. This paper outlines a practical reverse-transcription polymerase chain reaction (RT-PCR) and sequencing strategy that can be used to generate RNA sequences encoding the VP1 (1D) region of FMDV. The protocol contains a panel of PCR and sequencing primers that can be selected to characterise genetically diverse isolates representing all seven FMDV serotypes. A list of sequences is also described, comprising prototype sequences for all proposed FMDV topotypes, in order to provide a framework for phylogenetic analysis. The technical details and prototype sequences provided in this paper can be employed by FMD Reference Laboratories and others in an approach to harmonise the molecular epidemiology of FMDV.