Prefusion F, Postfusion F, G Antibodies, and Disease Severity in Infants and Young Children With Acute Respiratory Syncytial Virus Infection
Prefusion F, Postfusion F, G Antibodies, and Disease Severity in Infants and Young Children With Acute Respiratory Syncytial Virus Infection
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DOI：
10.1093/infdis/jix489
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发表时间：
2017-12-01
影响因子：
6.4
通讯作者：
Peeples, Mark E.
中科院分区：
文献类型：
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作者：
Capella, Cristina;Chaiwatpongsakorn, Supranee;Peeples, Mark E.
背景。呼吸道合胞病毒（RSV）是婴儿下呼吸道感染的最常见原因。母体衍生的 RSV 特异性抗体在预防生命早期 RSV 感染方面发挥着作用，但有关这些抗体的浓度和特异性的数据不完整。方法。我们前瞻性地招募了一组先前健康的因 RSV 感染住院的婴儿和幼儿 (n = 45) 或作为门诊患者 (n = 20) 进行评估，以及年龄匹配的健康未感染对照 (n = 18)。在入组时获取血清样本，以量化血清免疫球蛋白 G 抗体对 RSV 融合前（F 前）、融合后（F 后）和 G 糖蛋白的浓度和中和活性。我们还评估了抗体浓度与临床疾病严重程度之间的关联。结果。急性 RSV 感染婴儿血清中 F 前抗体的浓度比 F 后抗体高 3 倍以上，比 G 抗体高 30 倍以上。抗体浓度和中和活性与年龄呈负相关。 F前抗体显示出最大的中和活性（55%-100%），其次是G（0%-45%）和F后抗体（0%-29%）。较高浓度的前 F 和 G 抗体（而非后 F 抗体）与较低的临床疾病严重程度评分相关。结论。针对前 F 的母体抗体，随后针对 G 的抗体，可以调节小婴儿 RSV 疾病的严重程度。
Background. Respiratory syncytial virus (RSV) is the most frequent cause of lower respiratory tract infection in infants. Maternally derived RSV-specific antibodies play a role in protection against RSV infection in early life, but data regarding the concentration and specificity of those antibodies are incomplete.Methods. We prospectively enrolled a cohort of previously healthy infants and young children hospitalized (n = 45) or evaluated as outpatients (n = 20) for RSV infection, and healthy noninfected age-matched controls (n = 18). Serum samples were obtained at enrollment to quantify the concentrations and neutralizing activity of serum immunoglobulin G antibodies to the RSV prefusion (pre-F), postfusion (post-F), and G glycoproteins. We also assessed the associations between antibody concentrations and clinical disease severity.Results. Concentrations of pre-F antibodies were >= 3-fold higher than post-F antibodies and >30-fold higher than G antibodies in serum from infants with acute RSV infection. Antibody concentrations and neutralizing activity inversely correlated with age. The pre-F antibodies displayed the greatest neutralizing activity (55%-100%), followed by G (0%-45%), and post-F (0%-29%) antibodies. Higher concentrations of pre-F and G antibodies, but not post-F antibodies, were associated with lower clinical disease severity scores.Conclusions. Maternal antibodies directed to pre-F, followed by antibodies directed to G, can modulate RSV disease severity in young infants.