Cell cycle progression of C3H 10T1/2 and 3T3 cells in the absence of an increase in c-myc RNA levels.
Cell cycle progression of C3H 10T1/2 and 3T3 cells in the absence of an increase in c-myc RNA levels.
复制标题
在 c-myc RNA 水平不增加的情况下，C3H 10T1/2 和 3T3 细胞的细胞周期进展。
DOI：
10.1093/carcin/9.1.17
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发表时间：
1988
期刊：
影响因子：
4.7
通讯作者：
Kennedy,AR
中科院分区：
文献类型：
--
作者：
Chang,JD;Kennedy,AR
通常，当汇合细胞受到刺激分裂时，它们的 c-mycRNA 水平会短暂增加 (1-5)。这使得许多研究人员相信 c-mycRNA 的增加与细胞增殖的控制和调节存在因果关系。然而，我们在此报告，在蛋白酶抑制剂抗痛剂存在下生长至汇合的受刺激循环的 C3H 10T½ 和 3T3 细胞中，未观察到 c-mycRNA 水平的升高。在 c-mycRNA 不增加的情况下，细胞继续从细胞周期的 G0/G1 期进展到 S 期；血清刺激后[3H]胸苷掺入的动力学与在血清刺激后c-mycRNA水平升高的细胞中观察到的动力学相当。我们的观察具有重要意义，因为它们表明在 DNA 合成之前发生的 c-mycRNA 的短暂上升与静止成纤维细胞中启动 DNA 合成的事件之间存在分离。正如普遍认为的那样，c-myc 可能不会在刺激非循环、静止细胞在细胞周期中发挥核心作用。
Normally, when confluent cells are stimulated to divide, they show a transient increase in c-mycRNA levels (1–5). This has led many investigators to believe that the rise in c-mycRNA is causally related to the control and regulation of cell proliferation. However, we report here that the rise in c-mycRNA levels is not observed in stimulated cycling C3H 10T½ and 3T3 cells that have heen grown to confluence in the presence of the protease inhibitor antipain. Cells continue to progress from the G0/G1phase to S phase of the cell cycle in the absence of an increase in c-mycRNA; the kinetics of [3H]thymidine incorporation after serum stimulation are comparable to those observed in cells in which c-mycRNA levels rise after serum stimulation. Our observations are of significance because they suggest a dissociation between the transient rise of c-mycRNA which occurs before DNA synthesis and the events that initiate DNA synthesis in quiescent fibroblasts; c-mycmay not play as central a role in stimulating noncycling, quiescent cells to progress through the cell cycle, as has been generally assumed.