MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae.
MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae.
复制标题
MOT2 编码基因表达的负调节因子，影响酿酒酵母中信息素响应基因的基础表达。
DOI：
10.1128/mcb.14.5.3139-3149.1994
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发表时间：
1994
影响因子：
5.3
通讯作者：
Errede,B
中科院分区：
文献类型：
--
作者：
Cade,RM;Errede,B
Pheromones induce haploid cells ofSaccharomyces cerevisiaeto differentiate into a mating-competent state. Ste11p is one of several protein kinases required to transmit the pheromone-induced signal and to maintain basal expression of certain mating-specific genes in the absence of pheromone stimulation. To identify potential regulators of Ste11p, we screened for suppressors that restored mating and basal transcriptional competence to a strain with a conditionally functional Ste11p. This screen uncovered a novel gene we callMOT2, for modulator of transcription. Amot2deletion mutation leads to modest increases in the basal amounts of mRNA for several pheromone-responsive genes. Yetmot2deletion does not affect the signal transmission activity of the pathway in either the presence or absence of pheromone stimulation. Therefore, we propose that Mot2p, directly or indirectly, represses basal transcription of certain mating-specific genes. Becausemot2deletion mutants also have a conditional cell lysis phenotype, we expect that Mot2p regulatory effects may be more global than for mating-specific gene expression.
