CORTISOL - CORTISONE INTERCONVERSION BY HUMAN DECIDUA IN RELATION TO PARTURITION - EFFECT OF TISSUE MANIPULATION ON 11-BETA-HYDROXYSTEROID DEHYDROGENASE-ACTIVITY
CORTISOL - CORTISONE INTERCONVERSION BY HUMAN DECIDUA IN RELATION TO PARTURITION - EFFECT OF TISSUE MANIPULATION ON 11-BETA-HYDROXYSTEROID DEHYDROGENASE-ACTIVITY
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DOI：
10.1677/joe.0.0930141
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发表时间：
1982-01-01
影响因子：
4
通讯作者：
TURNBULL, AC
中科院分区：
文献类型：
--
作者：
BERNAL, AL;ANDERSON, ABM;TURNBULL, AC
在分娩开始前（择期剖宫产）、自然分娩后和引产后3组足月（37-42周）患者的蜕膜中研究了皮质醇：皮质醇的相互转化。当完整组织与[3 H]皮质醇或[3 H]可的松在磷酸盐缓冲液中孵育时，不添加底物或辅因子，可的松向皮质醇的转化占主导地位。然而，当在相同条件下使用受损细胞或组织匀浆时，反应的主导方向被逆转，氧化（皮质醇到可的松）活性大幅增加。在使用完整组织或匀浆的3组样品中，皮质醇：皮质醇的相互转化是相似的，总的11 β-皮质醇：皮质醇的相互转化也是相似的。羟基类固醇脱氢酶（11 β-在加入底物（皮质醇或可的松）和辅因子（NADP+或NADPH）的情况下，在组织匀浆中测量HSD）活性。蜕膜中内源性皮质醇浓度高于皮质醇浓度，且皮质醇与皮质醇的比值在3组中相似。显然，人类蜕膜11 β没有变化。HSD活动与分娩有关。11 β的比活性HSD在高蛋白浓度下降低，表明匀浆蜕膜组织中存在某些酶抑制剂。
Cortisol:cortisone interconversion was studied in human decidua obtained from 3 groups of patients at term (37-42 wk): before the onset of labor (at elective Cesarean section), after labor of spontaneous onset, and after labor of induced onset. When intact tissue was incubated with [3H]cortisol or [3H]cortisone in phosphate buffer without added substrate or cofactors, cortisone to cortisol was the dominant conversion. However, when damaged cells or tissue homogenates were used in the same conditions, the dominant direction of the reaction was reversed, with a large increase in oxidative (cortisol to cortisone) activity. Cortisol: cortisone interconversion was similar in the 3 groups of samples using either intact tissue or homogenates, as was the total 11.beta.-hydroxysteroid dehydrogenase (11.beta.-HSD) activity measured in tissue homogenates in the presence of added substrate (cortisol or cortisone) and cofactors (NADP+ or NADPH). Endogenous cortisol concentrations in decidua were higher than those of cortisone, and the ratio of cortisol to cortisone was similar in the 3 groups. Apparently, there are no changes in human decidual 11.beta.-HSD activity in relation to parturition. Specific activity of 11.beta.-HSD decreased at high protein concentrations, suggesting the presence of some enzyme inhibitor(s) in homogenized decidual tissue.