Multipotent mesenchymal stromal cells are fully permissive for human cytomegalovirus infection
Multipotent mesenchymal stromal cells are fully permissive for human cytomegalovirus infection
复制标题
多能间充质基质细胞完全允许人类巨细胞病毒感染
DOI：
10.1007/s12250-016-3754-0
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发表时间：
2016-04
影响因子：
5.5
通讯作者：
Min-Hua Luo
中科院分区：
文献类型：
--
作者：
Guan-Hua Qiao;Fei Zhao;Shuang Cheng;Min-Hua Luo
先天性人巨细胞病毒（HCMV）感染是导致出生缺陷的主要原因。先前的研究报告先天性HCMV感染的新生儿存在多器官发育不良的出生缺陷。多能间充质基质细胞（Multipotent mesenchymal stromal cells，MSCs）是一类具有自我更新能力的干/祖细胞，在多器官形成中起着重要作用。MSC是否易受HCMV感染尚不清楚。在这项研究中，间充质干细胞分离从沃顿氏胶的人脐带和鉴定其塑料粘附，表面标记图案，和分化能力。然后，用HCMV Towne株感染MSC，并通过使用蛋白质印迹、免疫荧光测定和空斑形成测定确定病毒进入、复制起始、病毒蛋白表达和感染性病毒体释放来评估感染状态。结果表明，所分离的MSCs对HCMV感染具有完全的耐受性，为了解HCMV感染在非神经系统疾病中的发病机制，包括胎儿发育过程中的多器官畸形提供了初步依据。
Congenital human cytomegalovirus (HCMV) infection is a leading infectious cause of birth defects. Previous studies have reported birth defects with multiple organ maldevelopment in congenital HCMV-infected neonates. Multipotent mesenchymal stromal cells (MSCs) are a group of stem/progenitor cells that are multi-potent and can self-renew, and they play a vital role in multi-organ formation. Whether MSCs are susceptible to HCMV infection is unclear. In this study, MSCs were isolated from Wharton’s jelly of the human umbilical cord and identified by their plastic adherence, surface marker pattern, and differentiation capacity. Then, the MSCs were infected with the HCMV Towne strain, and infection status was assessed via determination of viral entry, replication initiation, viral protein expression, and infectious virion release using western blotting, immunofluorescence assays, and plaque forming assays. The results indicate that the isolated MSCs were fully permissive for HCMV infection and provide a preliminary basis for understanding the pathogenesis of HCMV infection in non-nervous system diseases, including multi-organ malformation during fetal development.
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影响因子：
10.9
作者：
Nasreen A. Bhumbra;P. Lewandowski;P. Lau;Marasri Sererat-;M. Satish;G. Nankervis
通讯作者：
Nasreen A. Bhumbra;P. Lewandowski;P. Lau;Marasri Sererat-;M. Satish;G. Nankervis
影响因子：
5.6
作者：
Dudakovic, Amel;Camilleri, Emily T.;Lewallen, Eric A.;Mcgee-Lawrence, Meghan E.;Riester, Scott M.;Kakar, Sanjeev;Montecino, Martin;Stein, Gary S.;Ryoo, Hyun-Mo;Dietz, Allan B.;Westendorf, Jennifer J.;Van Wijnen, Andre J.
通讯作者：
Van Wijnen, Andre J.
影响因子：
3.7
作者：
M. Penfold;E. Mocarski
通讯作者：
M. Penfold;E. Mocarski
影响因子：
5.4
作者：
Luo, Min Hua;Fortunato, Elizabeth A.
通讯作者：
Fortunato, Elizabeth A.
DOI：
10.1089/clo.2008.0070
发表时间：
2009-03-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
Mrugala, Dominique;Dossat, Nadege;Jorgensen, Christian
通讯作者：
Jorgensen, Christian