Acute stress increases neuropsin mRNA expression in the mouse hippocampus through the glucocorticoid pathway
Acute stress increases neuropsin mRNA expression in the mouse hippocampus through the glucocorticoid pathway
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DOI：
10.1016/j.neulet.2008.03.042
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发表时间：
2008-05-09
影响因子：
2.5
通讯作者：
Komai, Shoji
中科院分区：
文献类型：
--
作者：
Harada, Akiko;Shiosaka, Sadao;Komai, Shoji
压力影响突触可塑性，并可能改变各种类型的行为，包括焦虑或记忆形成。在本研究中，我们研究了急性应激（1小时限制或不尾休克）的可塑性相关的丝氨酸蛋白酶neuropsin（NP）在海马的mRNA水平的影响，采用半定量RT-PCR和原位杂交。我们发现，NP mRNA表达显着增加后不久，暴露于急性束缚尾休克应激，并保持在高水平至少24小时。NP mRNA水平与血浆糖皮质激素皮质酮（CORT）浓度升高和应激强度相关。将CORT应用于原代培养的海马神经元（5 nM）或体内应用于肾上腺切除（ADX）小鼠（10 mg/kg BW，s.c.）模拟应激的作用，并显著升高NP mRNA。这些结果表明，应激后NP mRNA的上调是CORT依赖性的，并指出neuropsin在应激诱导的神经元可塑性中的作用。(c)2008爱思唯尔爱尔兰有限公司保留所有权利。
Stress affects synaptic plasticity and may alter various types of behaviour, including anxiety or memory formation. In the present study, we examined the effects of acute stress (1 h restraint with or without tail-shock) on mRNA levels of a plasticity-related serine protease neuropsin (NP) in the hippocampus using semiquantitative RT-PCR and in situ hybridization. We found that NP mRNA expression was dramatically increased shortly after exposure to the acute restraint tail-shock stress and remained at high level for at least 24 h. The level of NP mRNA would be correlated to the elevated plasma concentration of the glucocorticoid corticosterone (CORT) and to the stress intensity. Application of CORT either onto primary cultured hippocampal neurons (5 nM) or in vivo to adrenalectomized (ADX) mice (10 mg/kg BW, s.c.) mimicked the effect of stress and significantly elevated NP mRNA. These results suggest that the upregulation of NP mRNA after stress is CORT-dependent and point to a role for neuropsin in stress-induced neuronal plasticity. (c) 2008 Elsevier Ireland Ltd. All rights reserved.