Development of antibodies against pneumococcal proteins α-enolase, immunoglobulin A1 protease, streptococcal lipoprotein rotamase A, and putative proteinase maturation protein A in relation to pneumococcal carriage and Otitis Media
Development of antibodies against pneumococcal proteins α-enolase, immunoglobulin A1 protease, streptococcal lipoprotein rotamase A, and putative proteinase maturation protein A in relation to pneumococcal carriage and Otitis Media
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DOI：
10.1016/j.vaccine.2004.01.042
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发表时间：
2004-07-29
期刊：
影响因子：
5.5
通讯作者：
Hermans, PWM
中科院分区：
文献类型：
--
作者：
Adrian, PV;Bogaert, DB;Hermans, PWM
表面相关肺炎球菌蛋白α -烯醇化酶（Eno）、免疫球蛋白A1蛋白酶（Iga）、链球菌脂蛋白轮状酶A （SlrA）和假定的蛋白酶成熟蛋白A （PpmA）有可能成为未来基于蛋白的抗肺炎球菌疫苗的候选物。这些蛋白的免疫原性在329名出生后两年的儿童中进行了研究。在首次记录的中耳炎发作期间，可获得151名儿童的急性期和恢复期血清。采用EIA法检测血清中Eno、Iga、SlrA和PpmA抗体浓度，分别在99%（300/302）、95%（288/302）、95%（288/302）和83%（251/302）中检测到。有和没有肺炎球菌接触史的儿童组之间或肺炎球菌接触类型之间没有统计学上的显著差异。尽管AOM后恢复期血清中抗体滴度的平均总体下降，但一些儿童对AOM的抗体滴度能够增加两倍以上。大多数抗体浓度升高的儿童出现在血清收集时定植有肺炎球菌的组中，但记录为先前没有接触过肺炎球菌。综上所述，SlrA、PpmA、Eno和Iga是在生命早期引发抗体反应的免疫原性蛋白。这些蛋白的抗体滴度与肺炎球菌携带或感染之间没有明显的相关性。据推测，这是由于共生细菌上存在交叉反应性表位。(C) 2004 Elsevier Ltd.版权所有。
Surface associated pneumococcal proteins alpha-enolase (Eno), immunoglobulin A1 protease (Iga), streptococcal lipoprotein rotamase A (SlrA), and putative proteinase maturation protein A (PpmA) have potential as candidates for future protein-based anti-pneumococcal vaccines. The immunogenicity of these proteins were studied in a cohort of 329 children during their first two years of life. During the first recorded episode of otitis media, acute and convalescent phase sera were available from 151 children. Concentrations of antibodies against Eno, Iga, SlrA and PpmA were measured by EIA and detected in 99% (300/302), 95% (288/302), 95% (288/302), and 83% (251/302) of the sera, respectively. There were no statistically significant differences between the groups of children with and without a history of pneumococcal contact or with respect to the type of pneumococcal contact. Despite a mean overall decrease in the antibody titers in the convalescent sera following AOM, several children were able to respond with a more than twofold increase in antibody titer in response to AOM. The majority of the children with increased antibody concentrations appeared in the groups, which were colonized with pneumococci at the time of serum collection, but were recorded as having no prior contact with pneumococci. In conclusion, SlrA, PpmA, Eno and Iga are immunogenic proteins that elicit antibody responses early in life. No significant correlation between antibody titers to these proteins and pneumococcal carriage or infection was found. Presumably, this results from the presence of cross-reactive epitopes on commensal bacteria. (C) 2004 Elsevier Ltd. All rights reserved.