Soluble fibrin formation in the mesangial area of IgA nephropathy.
Soluble fibrin formation in the mesangial area of IgA nephropathy.
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DOI：
10.1007/s10157-006-0457-0
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发表时间：
2007-03-01
影响因子：
2.3
通讯作者：
Ono, Takahiko
中科院分区：
文献类型：
--
作者：
Liu, Ning;Mori, Noriko;Ono, Takahiko
背景：血栓形成的早期阶段血液中就发现了凝血素单体及其衍生物。当它们在血液中产生时，它们与纤维蛋白原形成复合物，并且它们以称为可溶性纤维蛋白（SF）的可溶性复合物形式存在。交联纤维蛋白（XFb）作为终末不溶性产物，常出现于活动型肾小球肾炎的系膜区。为探讨IgA肾病（IgAN）系膜SF产生的机制及其与XFb沉积的关系，采用免疫组化方法对19例IgAN患者进行研究。使用抗D-二聚体抗体结合纤溶酶暴露在肾活检标本中检测到XFb。SF用单克隆抗体（IF-43）检测，因子V用特异性兔抗体检测。SF染色的疾病活动指数，XFb沉积，和因子V染色的关系进行了evaluated.Results：XFb，因子V，和SF中观察到的系膜14，11，和8，共19个标本。SF在增殖区表达频繁，与XFb、F V表达有显著相关性（P <0.05）。此外，XFb，因子V，SF沉积显着相关性疾病活动（P <0.001，在每一个情况下）。结论：这些研究结果表明，SF是在肾小球系膜区形成的活动性IgA肾病伴肾小球系膜增生，特别是在其早期阶段。
BACKGROUND: Fibrin monomer and its derivatives in blood are found in an early stage of thrombosis. When they are produced in blood, they form complexes with fibrinogen, and they exist as soluble complexes named soluble fibrin (SF). As final insoluble products, cross-linked fibrin (XFb) is often observed in mesangial areas in active types of human glomerulonephritis. To clarify the mechanisms of mesangial SF production and its relationship to XFb deposition in IgA nephropathy (IgAN), an immunohistochemical study was conducted.METHODS: Nineteen patients with IgAN were studied. XFb was detected in renal biopsy specimens using anti-d-dimer antibody combined with plasmin exposure. SF was detected with a monoclonal antibody (IF-43), and factor V was detected with a specific rabbit antibody. The relationships of SF staining to the disease activity index, XFb deposition, and factor V staining was evaluated.RESULTS: XFb, factor V, and SF were observed in the mesangium in 14, 11, and 8, respectively, of a total of 19 specimens. SF had frequent staining in the proliferating areas, showing a significant relationship to XFb or factor V (P < 0.05). Furthermore, XFb, factor V, and SF depositions were markedly correlated with disease activity (P < 0.001 in each case).CONCLUSIONS: These findings suggest that SF is formed in the mesangial area in active IgA nephropathy accompanied by mesangial proliferation, in particular, in its early stage.