Animal models for the assessment of novel vascular conduits
Animal models for the assessment of novel vascular conduits
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DOI：
10.1016/j.jvs.2009.10.080
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发表时间：
2010-07-01
影响因子：
4.3
通讯作者：
Ng, Martin K. C.
中科院分区：
文献类型：
--
作者：
Byrom, Michael J.;Bannon, Paul G.;Ng, Martin K. C.
用于血管搭桥手术的理想小直径管道的开发尚未实现。正在进行的生物材料设计创新产生了需要在体内进行临床前评估的新管道，许多动物模型已用于此目的。这篇文章考察了在评估小直径血管管道时使用动物模型的基本原理，这些血管管道涵盖了常用的物种：狒狒、绵羊、猪、狗、兔子和啮齿动物。总结了这些物种相对于人类的血液学比较研究，并比较了常见植入部位的流体动力学数值。然后探索大动物模型和小动物模型，突出每种模型的特征，这些特征决定了它们在评估血管导管中的相对效用。在可能的情况下，给出了每种动物和每个地点的膨化聚四氟乙烯的性能，以便在不同物种之间进行直接比较。为评估组织工程移植物设计，概述了动物建模方面的新挑战。最后，对未来评估血管管道的动物模型的选择提出了建议。(J Vase Surg 2010；52：176-95.)临床相关性：血管外科中使用的自体动脉和静脉节段的局限性推动了新血管管道的发展，最终需要在体内进行评估。大量宣称成功开发新管道的文章与少数进入临床试验的候选项目形成了鲜明对比，这种差异至少部分是因为在选择和使用适当的动物模型方面缺乏标准化的方法。这篇综述试图首次对决定用于血管管道评估的物种的实用性的相关变量进行广泛的比较，这与研究人员和临床医生评估新管道发展的报告有关。
The development of an ideal small-diameter conduit for use in vascular bypass surgery has yet to be achieved. The ongoing innovation in biomaterial design generates novel conduits that require preclinical assessment in vivo, and a number of animal models have been used for this purpose. This article examines the rationale behind animal models used in the assessment of small-diameter vascular conduits encompassing the commonly used species: baboons, sheep, pigs, dogs, rabbits, and rodents. Studies on the comparative hematology for these species relative to humans are summarized, and the hydrodynamic values for common implant locations are also compared. The large- and small-animal models are then explored, highlighting the characteristics of each that determine their relative utility in the assessment of vascular conduits. Where possible, the performance of expanded polytetrafluoroethylene is given in each animal and in each location to allow direct comparisons between species. New challenges in animal modeling are outlined for the assessment of tissue-engineered graft designs. Finally, recommendations are given for the selection of animal models for the assessment of future vascular conduits. ( J Vase Surg 2010;52:176-95.)Clinical Relevance: The limitations of autologous arterial and venous segments used in vascular surgery drives the development of novel vascular conduits which ultimately require assessment in vivo. The abundance of articles proclaiming successful development of new conduits contrasts starkly with the handful of candidates reaching clinical trials, a discrepancy due at least in part to a lack of standardised approach to the selection and utilisation of appropriate animal models. This review seeks to provide for the first time a broad comparison of the pertinent variables determining the utility of species used for vascular conduit assessment, relevant to researchers and to clinicians evaluating reports of novel conduit development.