Mechanism of action of hemolysin III from Bacillus cereus
Mechanism of action of hemolysin III from Bacillus cereus
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DOI：
10.1016/s0005-2736(96)00168-x
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发表时间：
1996-10-23
影响因子：
3.4
通讯作者：
Kuzmin, NP
中科院分区：
文献类型：
--
作者：
Baida, GE;Kuzmin, NP
在携带 hly-III 基因的大肠杆菌粗提物中测试了蜡样芽孢杆菌溶血素 III 活性。结论溶血素III是一种成孔溶血素，其功能孔径约为3-3.5 nm。溶血至少发生在三个步骤中：（i）HIV-III单体与红细胞膜的温度依赖性结合；（ii）跨膜寡聚孔的温度依赖性形成； (iii) 与温度无关的红细胞裂解。
Bacillus cereus hemolysin III activity was tested in crude extracts from Escherichia coli carrying the hly-III gene. It was concluded that hemolysin III is a pore-forming hemolysin with a functional pore diameter of about 3-3.5 nm. Hemolysis occurs in at least three steps: (i) the temperature-dependent binding of the HIV-III monomers to the erythrocyte membrane: (ii) the temperature-dependent formation of the transmembrane oligomeric pore; (iii) the temperature-independent erythrocyte lysis.