A Combination of Resveratrol and Quercetin Induces Browning in White Adipose Tissue of Rats Fed an Obesogenic Diet
A Combination of Resveratrol and Quercetin Induces Browning in White Adipose Tissue of Rats Fed an Obesogenic Diet
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DOI：
10.1002/oby.21706
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发表时间：
2017-01-01
期刊：
影响因子：
6.9
通讯作者：
Portillo, Maria P.
中科院分区：
文献类型：
--
作者：
Arias, Noemi;Pico, Catalina;Portillo, Maria P.
目的：为了分析槲皮素（Q）和白藜芦醇（RSV）的组合是否会诱导白色脂肪组织（WAT）的布朗宁效应。（15 mg/kg体重/天; RSV组），用Q结果：6周后，RSV + Q组的体重和WAT显著降低。在对照组、RSV组和Q组的肾周WAT中，大多数细胞中出现白色单室脂肪细胞，而在RSV + Q组中观察到许多UCP 1免疫染色阳性的多室脂肪细胞。通过Western blot证实UCP 1的存在。该组还显示Cidea、Hocx 9、Bmp 4、Slc 27 a1、Pat 2、Atgl和Atp 5d的mRNA水平增加。肩胛间棕色脂肪组织重量在组间无差异，但RSV组中Cidea mRNA水平增加，RSV 1 Q组中考克斯-2 mRNA水平增加，RSV和RSV + Q组中UCP 1蛋白表达增加。这项研究表明，RSV + Q组合在肾周WAT中产生棕色样重塑效应，以及肩胛间棕色脂肪组织中UCP 1蛋白表达增加。
Objective: To analyze whether a combination of quercetin (Q) and resveratrol (RSV) would induce a white adipose tissue (WAT) browning effect.Methods: Thirty-six rats were fed an obesogenic diet and divided into four groups: control, treated with RSV (15 mg/kg body weight/day; RSV group), treated with Q (30 mg/kg body weight/day; Q group), or treated with both polyphenols (RSV + Q group).Results: After 6 weeks, body and WAT weights were significantly reduced in the RSV + Q group. In perirenal WAT of the control, RSV, and Q groups, white unilocular adipocytes appeared in the majority of cells, while in the RSV + Q group numerous multilocular adipocytes with positive immunostaining for UCP1 were observed. The presence of UCP1 was confirmed by Western blot. This group also revealed increased mRNA levels of Cidea, Hocx9, Bmp4, Slc27a1, Pat2, Atgl, and Atp5d. Interscapular brown adipose tissue weight showed no differences between groups, but the Cidea mRNA level was increased in the RSV group, the Cox-2 mRNA level in the RSV1Q group, and UCP1 protein expression in the RSV and the RSV + Q groups.Conclusions: This study demonstrated that the RSV + Q combination produces a brown-like remodeling effect in perirenal WAT, as well as increased UCP1 protein expression in interscapular brown adipose tissue.