Th1 and Th2 cytokine production is suppressed at the level of transcriptional regulation in Kawasaki disease
Th1 and Th2 cytokine production is suppressed at the level of transcriptional regulation in Kawasaki disease
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DOI：
10.1111/j.1365-2249.2004.02506.x
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发表时间：
2004-08-01
影响因子：
4.6
通讯作者：
Hara, T
中科院分区：
文献类型：
--
作者：
Kimura, J;Takada, H;Hara, T
为了阐明川崎病T细胞的功能状态，我们分析了Th1/Th2细胞因子(干扰素-γ和IL-4)以及Th1/Th2诱导转录因子T-bet和GATA-3的mRNA表达水平，它们分别在Th1和Th2细胞的发育中发挥关键作用。实时荧光定量聚合酶链式反应检测显示，川崎病患者外周血单个核细胞(PBMNC)中干扰素-γ的表达水平明显低于麻疹患者，且有降低的趋势。川崎病患者外周血中T-bet基因表达水平明显低于正常对照组。川崎病患者外周血中IL-4和GATA-3mRNA水平较正常对照组显著降低。川崎病患者外周血中调节性细胞因子mRNA(转化生长因子-β和IL-10)水平也降低。川崎病患者外周血中干扰素-γ的表达水平与T-bet的表达呈显著正相关。提示Th1和Th2功能受抑与T-bet和GATA-3基因表达受抑有关，可能是川崎病的免疫学特征之一。
To clarify the functional state of T cells in Kawasaki disease, we analysed mRNA expression levels of Th1/Th2 cytokines (IFN-gamma and IL-4) along with Th1/Th2-inducing transcription factors, T-bet and GATA-3, which play pivotal roles in the development of Th1 and Th2 cells, respectively. By real-time PCR, IFN-gamma mRNA levels in peripheral blood mononuclear cells (PBMNC) were significantly decreased in Kawasaki disease patients compared with those with measles, and tended to be lower than those in healthy controls. T-bet mRNA levels were significantly decreased in patients with Kawasaki disease compared with healthy controls. In addition, IL-4 and GATA-3 mRNA levels were significantly decreased in Kawasaki disease compared with healthy controls. Regulatory cytokine mRNA levels (TGF-beta and IL-10) were also decreased in Kawasaki disease. The mRNA levels of IFN-gamma showed a significant positive correlation with those of T-bet in Kawasaki disease. These results suggest that the suppressed function of Th1 and Th2, associated with the suppression of both T-bet and GATA-3 gene expression, may be one of the immunological characteristics of Kawasaki disease.