Campylobacter jejuni strains of human and chicken origin are invasive in chickens after oral challenge
Campylobacter jejuni strains of human and chicken origin are invasive in chickens after oral challenge
复制标题
DOI：
10.1637/7376-051005r.1
复制
发表时间：
2006-03-01
期刊：
影响因子：
1.4
通讯作者：
Madsen, M
中科院分区：
文献类型：
--
作者：
Knudsen, KN;Bang, DD;Madsen, M
本研究旨在评价选定的对丹麦肉鸡空肠弯曲杆菌流行病学有重要意义的空肠弯曲杆菌菌株的定殖能力和侵袭能力。选取4株C型空肠菌株，在SPF蛋孵化的1日龄和14日龄鸡中进行了实验定植研究。在检测的四种C型空肠菌株中，有三种是Penner热稳定血清型2，flaA型1/1，这是丹麦肉鸡和人类病例中最常见的类型。第四种毒株是Penner热稳定血清型19，已被证明与人类格林-巴利综合征（GBS）有关。在粘土龄鸡中建立定殖的最低剂量约为2 cfu，而在14日龄鸡中建立定殖需要2至3倍的剂量。其中两种空肠C菌株在口腔攻毒鸡以及三种不同的人类上皮细胞系中具有侵袭性。
The aim of the study was to evaluate the colonizing ability and the invasive capacity of selected Campylobacter jejuni strains of importance for the epidemiology of C jejuni in Danish broiler chickens. Four C jejuni strains were selected for experimental colonization Studies in day-old and 14-day-old chickens hatched from specific pathogen free (SPF) eggs. Of the four C jejuni strains tested, three were Penner heat-stable serotype 2,flaA type 1/1, the most common type found among broilers and human cases in Denmark. The fourth strain was Penner heat-stable serotype 19, which has been shown to be associated with the Guillain Barre Syndrome (GBS) in humans. The minimum dose for establishing colonization in the clay-old chickens was approximately 2 cfu, whereas two- to threefold higher doses were required for establishing colonization in the 14-day-old chickens. Two of the C jejuni strains were shown to be invasive in orally challenged chickens as well as in three different human epithelial cell lines.