Induction of pancreatic differentiation by signals from blood vessels
Induction of pancreatic differentiation by signals from blood vessels
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DOI：
10.1126/science.1064344
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发表时间：
2001-10-19
期刊：
影响因子：
56.9
通讯作者：
Melton, D
中科院分区：
文献类型：
--
作者：
Lammert, E;Cleaver, O;Melton, D
血管为发育中的器官提供代谢所需的营养物质。在此，我们证明了血管在胰腺器官发生过程中作为发育信号源的作用。对胚胎小鼠组织进行的体外实验表明，血管内皮可诱导分离的内胚层表达胰岛素。在非洲爪蟾胚胎中去除背主动脉会导致体内胰岛素表达失败。此外，通过使用转基因小鼠，我们发现前肠后部的异位血管形成会导致胰岛素异位表达和胰岛增生。这些结果表明，血管不仅提供代谢所需的营养物质，还为器官发育提供诱导信号。
Blood vessels supply developing organs with metabolic sustenance. Here, we demonstrate a role for blood vessels as a source of developmental signals during pancreatic organogenesis. In vitro experiments with embryonic mouse tissues demonstrate that blood vessel endothelium induces insulin expression in isolated endoderm. Removal of the dorsal aorta in Xenopus laevis embryos results in the failure of insulin expression in vivo. Furthermore, using transgenic mice, we show that ectopic vascularization in the posterior foregut leads to ectopic insulin expression and islet hyperplasia. These results indicate that vessels not only provide metabolic sustenance, but also provide inductive signals for organ development.