Quartz crystal microbalance with dissipation monitoring and the real-time study of biological systems and macromolecules at interfaces
Quartz crystal microbalance with dissipation monitoring and the real-time study of biological systems and macromolecules at interfaces
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DOI：
10.3233/bsi-2012-0025
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Gomez-Fernandez, Juan C.
中科院分区：
文献类型：
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作者：
Ausili, Alessio;Berglin, Mattias;Gomez-Fernandez, Juan C.
QCM-D技术基于在石英中产生具有振荡共振的声剪切波的物理现象，从而导致在石英和溶液的界面处产生倏逝波。声波的振幅受材料沉积到石英表面上的影响，并且从随后的频率降低可以计算出束缚质量。所产生的耗散移位告知被吸附材料的粘弹性和柔性。由于QCM-D技术简单、快速、低成本、灵敏度高，不需要对样品进行标记，因此可用于多种生物体系的实时研究。在生物领域中的常见应用包括直接测量脂质、蛋白质、DNA和细胞在传感器表面上的吸附，这些传感器通常通过自组装单层（SAM）技术或通过旋涂聚合物进行化学修饰。QCM-D还可用于研究大分子与吸附物质之间的分子相互作用。使用这种技术的三个例子，即对接取向的C2结构域的PKC β磷脂膜，吸附到模型丙烯酸聚合物的纤维蛋白原的构象变化和附着的内皮细胞的羧化聚合物的不同配置。
QCM-D technique is based on the physical phenomenon that generates an acoustic shear wave with an oscillating resonance in quartz resulting in an evanescent wave that arises at the interface of the quartz and the solution. The amplitude of the acoustic wave is influenced by the deposition of material onto the quartz surface and from the subsequent decrease of the frequency the bound mass can be calculated. The dissipation shift which arises inform about viscoelasticity and flexibility of the adsorbed material. QCM-D can be applied for real-time studies of several biological systems since it is a simple, fast, low-cost and sensitive technique without having to label any sample. Common applications in biological field include measurements on adsorption of lipids, proteins, DNA and cells directly onto the surface of the sensor, which generally are chemically modified by self-assembled monolayer (SAM) technique or by spin-coated polymers. QCM-D can also be used to study molecular interactions between macromolecules and adsorbed materials. Three examples of the use of this technique are presented, namely the docking orientation of the C2 domain of PKC epsilon on phospholipid membranes, the conformational changes of fibrinogen adsorbed to model acrylic polymers and the attachment of endothelial cells to carboxylated polymers of different configuration.