Peroxidase-dependent metal-independent oxidation of low density lipoprotein in vitro: a model for in vivo oxidation?
Peroxidase-dependent metal-independent oxidation of low density lipoprotein in vitro: a model for in vivo oxidation?
复制标题
低密度脂蛋白的过氧化物酶依赖性金属非依赖性氧化体外：体内氧化模型？
DOI：
10.1073/pnas.90.13.5929
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发表时间：
1993
影响因子：
11.1
通讯作者：
Steinberg,D
中科院分区：
文献类型：
--
作者：
Wieland,E;Parthasarathy,S;Steinberg,D
低密度脂蛋白的氧化修饰被认为是脂蛋白致动脉粥样硬化的重要途径。到目前为止所描述的低密度脂蛋白的体外氧化修饰系统似乎都依赖于介质中自由过渡金属离子(铜或铁)的存在。另一方面，在体内，这些金属几乎完全以紧密络合的形式存在，不催化氧化修饰。目前的研究描述了在一个简单的体系中氧化低密度脂蛋白，该体系不依赖于添加的自由金属离子的存在。它需要辣根过氧化物酶和过氧化氢或脂质过氧化氢的存在。
Oxidative modification of low density lipoprotein is believed to be an important pathway by which the lipoprotein becomes atherogenic. The in vitro systems for oxidative modification of low density lipoprotein thus far described all appear to depend upon the presence in the medium of free transition metal ions (copper or iron). In vivo, on the other hand, these metals are present almost exclusively in tightly complexed forms that do not catalyze oxidative modification. The present studies describe oxidation of low density lipoprotein in a simple system that does not depend upon the presence of added free metal ions. It requires the presence of horseradish peroxidase and either hydrogen peroxide or lipid hydroperoxides.