ARREST OF MAMMARY-TUMOR GROWTH-INVIVO BY L-ARGININE - STIMULATION OF NAD-DEPENDENT ACTIVATION OF ADENYLATE-CYCLASE
ARREST OF MAMMARY-TUMOR GROWTH-INVIVO BY L-ARGININE - STIMULATION OF NAD-DEPENDENT ACTIVATION OF ADENYLATE-CYCLASE
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DOI：
10.1016/0006-291x(80)91616-2
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
HILL, DM
中科院分区：
文献类型：
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作者：
CHOCHUNG, YS;CLAIR, T;HILL, DM
每日注射L精氨酸(L-精氨酸)可抑制荷尔蒙依赖性大鼠乳腺肿瘤的生长。盐酸50 mg/200g大鼠皮下注射)。精氨酸+N_6，O_2‘-二丁酰cAMP(DBcAMP)协同作用增强细胞生长抑制作用。精氨酸对细胞生长的抑制伴随着细胞内cAMP含量的急剧增加，在此之前，膜蛋白依赖于NAD的ADP核糖化和依赖于NAD的腺苷环化酶的激活平行增加。膜蛋白的ADP-核糖化需要GTP，同样由105,000克肿瘤上清液部分和霍乱毒素催化。精氨酸在cAMP介导的抑制乳腺肿瘤中的特殊作用被提出。
In vivo growth of hormone-dependent rat mammary tumors was arrested by daily injections of L-arginine (L-arginine .cntdot. HCl 50 mg/200 g rat s.c.). Arginine + N6,O2''-dibutyryl cAMP (DBcAMP) acted synergistically to enhance the growth inhibitory effect. Growth arrest by arginine was accompanied by a sharp increase in cellular cAMP content, which was preceded by parallel increases in NAD-dependent ADP-ribosylation of the membrane proteins and NAD-dependent activation of adenylate cyclase. The ADP-ribosylation of the membrane proteins required GTP and was catalyzed similarly by the 105,000 g supernatant fraction of the tumor and by cholera toxin. A specific role for arginine in the cAMP-mediated inhibition of manmary tumors is suggested.