Marrow stimulation and chondrocyte transplantation using a collagen matrix for cartilage repair
Marrow stimulation and chondrocyte transplantation using a collagen matrix for cartilage repair
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DOI：
10.1016/j.joca.2005.04.001
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发表时间：
2005-08-01
影响因子：
7
通讯作者：
Nehrer, S
中科院分区：
文献类型：
--
作者：
Dorotka, R;Bindreiter, U;Nehrer, S
目的：该研究的目的是确定支架的植入是否会促进绵羊微骨折后12个月的软骨修复。此外，我们研究了额外的自体细胞扩增的植入constructs.Methods的效果：两个软骨缺损产生在内侧股骨髁的羊没有穿透软骨下骨。将27只绵羊分为以下组：7只作为未处理对照组（第1组），在20只动物中创建微骨折，其中7只未进行进一步处理（第2组），在6只绵羊中，用猪胶原基质额外覆盖缺损（第3组），在7只动物中，用培养的自体软骨细胞增强基质（第4组）。经过4个月（11只羊）和12个月（16只羊），填充的缺陷，组织类型，和半定量scores.Results：未经处理的缺陷显示最少量的缺陷填充。用微骨折治疗的缺损获得了更好的缺损填充，而额外使用基质并没有增加缺损填充。在细胞增强组中发现了最大量的修复组织。半定量评分最好的细胞增强group.Conclusion：微骨折治疗观察到，以提高愈合反应。细胞接种基质的植入进一步改善了结果。单独植入胶原基质不能增强修复。自体干细胞移植是软骨缺损组织工程治疗的一个重要方面。(C)2005年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: The purpose of the study was to determine whether the implantation of a scaffold would facilitate cartilage repair after microfracture in sheep over a period of 12 months. Furthermore, we investigated the effect of additional autologous cell augmentation of the implanted constructs.Methods: Two chondral defects were produced in the medial femoral condyle of sheep without penetrating the subchondral bone. Twenty-seven sheep were divided into the following groups: seven served as untreated controls (Group 1), microfracture was created in 20 animals, seven of them without further treatment (Group 2), in six sheep the defects were additionally covered with a porcine collagen matrix (Group 3), and in seven animals the matrix was augmented with cultured autologous chondrocytes (Group 4). After 4 (11 sheep) and 12 months (16 sheep), the filling of the defects, tissue types, and semiquantitative scores were determined.Results: The untreated defects revealed the least amount of defect fill. Defects treated with microfractures achieved better defect fill, while the additional use of the matrix did not increase the defect fill. The largest quantity of reparative tissue was found in the cell-augmented group. Semiquantitative scores were best in the cell-augmented group.Conclusion: Microfracture treatment was observed to enhance the healing response. The implantation of a cell-seeded matrix further improved the outcome. The implantation of a collagen matrix alone did not enhance repair. Autologous cell implantation appears to be a very important aspect of the tissue engineering approach to cartilage defects. (C) 2005 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.