Separation of polyethylene glycols and amino-terminated polyethylene glycols by high-performance liquid chromatography under near critical conditions
Separation of polyethylene glycols and amino-terminated polyethylene glycols by high-performance liquid chromatography under near critical conditions
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近临界条件下高效液相色谱分离聚乙二醇和氨基封端聚乙二醇
DOI：
10.1016/j.chroma.2016.04.035
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发表时间：
2016
影响因子：
4.1
通讯作者：
Jiang X. -L.
中科院分区：
文献类型：
--
作者：
Wei Y. -Z.;Zhuo R. -X.;Jiang X. -L.
介绍了聚乙二醇及其氨基取代衍生物在普通硅胶反相填充柱上等度洗脱的分离和表征。这种分离是通过近临界条件下的液相色谱法（LCCC）实现的，基于氨基官能端基的数量，而PEG的摩尔质量没有明显的影响。移动的流动相为乙腈水溶液和最佳醋酸铵缓冲液。PEG和氨基取代PEG在硅胶柱上的近液相色谱分离机理为离子交换作用。在PEG骨架的LCCC条件下，通过微调缓冲液浓度，以苄胺和苯酚在缓冲移动的相中的保留因子比α（苄胺/苯酚）值来评价硅胶基反相填充柱的离子交换容量。据我们所知，这是第一次报告分离氨基功能PEG的摩尔质量独立的等度洗脱使用常见的C18或苯基反相填充柱。
The separation and characterization of polyethylene glycols (PEGs) and amino-substituted derivatives on common silica-based reversed-phase packing columns using isocratic elution is described. This separation is achieved by liquid chromatography under the near critical conditions (LCCC), based on the number of amino functional end groups without obvious effect of molar mass for PEGs. The mobile phase is acetonitrile in water with an optimal ammonium acetate buffer. The separation mechanism of PEG and amino-substituted PEG under the near LCCC on silica-based packing columns is confirmed to be ion-exchange interaction. Under the LCCC of PEG backbone, with fine tune of buffer concentration, the retention factor ratios for benzylamine and phenol in buffered mobile phases, α(benzylamine/phenol)-values, were used to assess the ion-exchange capacity on silica-based reversed-phase packing columns. To the best of our knowledge, this is the first report on separation of amino-functional PEGs independent of the molar mass by isocratic elution using common C18 or phenyl reversed-phase packing columns.