Changes in the TNF-alpha/IL-10 ratio in hyperglycemia-associated pregnancies
Changes in the TNF-alpha/IL-10 ratio in hyperglycemia-associated pregnancies
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DOI：
10.1016/j.diabres.2015.01.005
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发表时间：
2015-03-01
影响因子：
5.1
通讯作者：
Calderon, Iracema M. P.
中科院分区：
文献类型：
--
作者：
Moreli, Jusciele B.;Correa-Silva, Simone;Calderon, Iracema M. P.
目的：肿瘤坏死因子-α是一种与妊娠期不良结局相关的糖尿病细胞因子，可被IL-10抵消。方法：192例孕妇分为正常血糖组、轻度妊娠高血糖组、妊娠期糖尿病组和2型糖尿病组，共192例。结果：妊娠期糖尿病孕妇血浆中肿瘤坏死因子-α水平最高(p=0.0190)，而糖尿病患者胎盘组织中肿瘤坏死因子-α水平最高(p=0.0095)。免疫组织化学显示DM2组绒毛结构中有较强的抗肿瘤坏死因子-α抗体反应。相反，DM2组母体血浆中IL-10水平最低(p=0.0228)。母血中肿瘤坏死因子-α/白介素10的比值随着高血糖的严重程度而逐渐升高(p<；0.0001)，在DM2组胎盘中最高(p=0.0150)。血浆和胎盘中TNF-α/IL-10比值与母体平均血糖和HbA1c水平呈正相关。结论：胎盘和血清中以TNF-α为主的平衡改变与妊娠期高血糖的严重程度相关。这种联系可能有助于了解妊娠期高血糖的发病机制和相关的妊娠结局。(C)2015爱思唯尔爱尔兰有限公司。保留所有权利。
Aims: TNF-alpha is a diabetogenic cytokine associated with adverse outcomes during pregnancy that can be counterbalanced by IL-10. We have investigated IL-10 and TNF-alpha balance at maternal and placental levels in hyperglycemia-associated pregnancies.Methods: One hundred and ninety-two pregnant women participated, which included normoglycemic women (ND) and women with mild gestational hyperglycemia (MGH), gestational diabetes mellitus (GDM) and type 2 diabetes mellitus (DM2). Maternal plasma and placental tissue IL-10 and TNF-alpha levels were measured by ELISA and placental TNF-alpha was also immunolocalized.Results: Maternal plasma TNF-alpha levels were highest in GDM (p = 0.0190), whereas TNF-alpha levels were highest in placental tissues in DM2 (p = 0.0095). Immunohistochemistry also showed strong reactivity with anti-TNF-alpha antibody in the villous structures in the DM2 group. Conversely, IL-10 levels were lowest in maternal plasma of the DM2 group (p = 0.0228). The TNF-alpha/IL-10 ratio in maternal plasma progressively increased with the severity of hyperglycemia (p < 0.0001), being highest in placenta of the DM2 group (p = 0.0150). In both, plasma and placenta, TNF-alpha/IL-10 ratio were correlated with mean maternal glycemia and HbA1c levels.Conclusions: Alterations of placenta and serum TNF-alpha/IL-10 balance with predominance of TNF-alpha were correlated with the severity of hyperglycemia during gestation. This association may offer insight into the pathogenesis of gestational hyperglycemia and associated pregnancy outcomes. (C) 2015 Elsevier Ireland Ltd. All rights reserved.