Analysis of the physicochemical properties, cytotoxicity and volumetric changes of AH Plus, MTA Fillapex and TotalFill BC Sealer.
Analysis of the physicochemical properties, cytotoxicity and volumetric changes of AH Plus, MTA Fillapex and TotalFill BC Sealer.
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分析AH Plus，MTA Fillapex和TotalFill BC密封剂的物理化学特性，细胞毒性和体积变化。
DOI：
10.4317/jced.57527
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发表时间：
2020-11
影响因子：
--
通讯作者：
Bernardineli N
中科院分区：
文献类型：
--
作者：
Almeida MM;Rodrigues CT;Matos AA;Carvalho KK;Silva EJ;Duarte MA;Oliveira RC;Bernardineli N
评价AH Plus、MTA Filltex和TotalFill BC封闭剂的理化性能和细胞毒性。使用微型计算机断层扫描(Micro-CT)评估体积变化。根据ISO 6876评估放射不透明度和流量，同时根据ASTM-C266-08规范评估凝结时间。在不同时间间隔(1h、3h、24h、72h、168h、360h)分别用分光光度计法和pH计测定细胞内钙离子的释放和pH。采用四甲基偶氮唑蓝还原比色法测定细胞毒作用，分别于24、48、72h检测3T3细胞活性。用Micro-CT对30颗充填牙胶尖和所测试的根管封闭剂的牙冠牙体的体积变化进行评价。在蒸馏水中浸泡168h、360h和720h后对样品进行评价。统计分析采用单因素方差分析和Tukey检验或Kruskal-Wallis和Dunn检验(P<；0.05)。MTA Filltex和TotalFill BC封闭剂的X线透过率均低于AH Plus(P<；0.05)。MTA Filltop的流量最高，AH Plus的流量最低(P<；0.05)。AH Plus封闭剂的初凝和终凝时间均低于MTA Filltex和TotalFill BC封闭剂(P<；0.05)。总的来说，TotalFill BC封闭剂的钙离子释放量和pH值均高于其他受试封闭剂。与其他封闭剂相比，TotalFill BC封闭剂显示出总体较低的细胞毒性。AH Plus和TotalFill BC封闭剂的体积变化小于MTA Filltex(P<；0.05)。AH Plus、MTA Filltex和TotalFill BC封闭剂在理化性质和细胞毒性方面略有差异，但均适合作为根管封闭剂。然而，AH Plus和TotalFill BC封闭剂与MTA Filltex相比显示出较低的体积变化。关键词：硅酸钙，细胞毒性，理化性质，显微计算机断层扫描。
To evaluate the physicochemical properties and cytotoxicity of AH Plus, MTA Fillapex and TotalFill BC Sealer. Volumetric changes were also evaluating using micro-computed tomography (micro-CT). Radiopacity and flow were evaluated in accordance with the ISO 6876, while setting time was evaluated in accordance with the ASTM- C266-08 specifications. The release of Ca2+ ions and pH were measured with spectrophotometer and pH meter, respectively, after different time intervals (1h, 3h, 24h, 72h, 168h, and 360h). Cytotoxicity was evaluated by MTT reduction assay to check 3T3 cells viability at 24, 48 and 72 hours. Volumetric change was evaluated by micro-CT, by using 30 acrylic teeth, filled with gutta-percha cones and the tested root canal sealer. The samples were evaluated after 168h, 360h and 720h of immersion in distilled water. Data were statistically analyzed by one-way ANOVA and Tukey test or by Kruskal-Wallis and Dunn tests (P<0.05). MTA Fillapex and TotalFill BC Sealer showed lower radiopacity than AH Plus (P<0.05). The MTA Fillapex showed the highest flow, while AH Plus showed the lowest flow (P<0.05). The initial and final setting time of AH Plus were lower than MTA Fillapex and TotalFill BC Sealer (P<0.05). In general, TotalFill BC Sealer presented higher Ca2+ ion release and pH than the other tested sealers. TotalFill BC Sealer also showed overall lower cytotoxicity when compared to the other sealers. Volumetric change of AH Plus and TotalFill BC Sealer was lower than MTA Fillapex (P<0.05). AH Plus, MTA Fillapex and TotalFill BC Sealer showed slight differences in the physicochemical properties and cytotoxicity, but all suitable for an endodontic sealer. However, AH Plus and TotalFill BC Sealer showed low volumetric changes when compared to MTA Fillapex. Key words:Calcium silicate, cytotoxicity, physicochemical properties, micro computed tomography.