Frequency- and duration-dependent effects of cyclic pressure on select bone cell functions
Frequency- and duration-dependent effects of cyclic pressure on select bone cell functions
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DOI：
10.1089/107632701753337672
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发表时间：
2001-12-01
影响因子：
--
通讯作者：
Bizios, R
中科院分区：
文献类型：
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作者：
Nagatomi, J;Arulanandam, BP;Bizios, R
目前的研究表明，独特的相关性之间的特征参数的机械负荷和成骨细胞的功能。具体而言，成骨细胞增殖依赖于频率和施加的周期性压力刺激的持续时间：只有当这些细胞暴露于1.0 Hz（但不是在0.25 Hz）频率的周期性压力1小时（但不是20分钟），每天5天时，才观察到细胞增殖减少。相比之下，内皮细胞对循环压力没有反应，而成纤维细胞增殖在类似的测试条件下增加。最重要的是，周期性压力影响不同的成骨细胞基因：暴露成骨细胞的周期性压力（在1.0赫兹的频率为1小时，每天）导致增强转录和翻译的碱性磷酸酶5天后，同样的机械刺激，但是，并没有影响骨桥蛋白mRNA的表达在相同的时间段。这些结果提供了细胞和分子水平的信息，这不仅是重要的阐明机械负荷和骨稳态之间的相关性，但可以在骨组织工程的新技术的开发是有用的。
The present study demonstrated unique correlations between characteristic parameters of mechanical loading and osteoblast functions. Specifically, osteoblast proliferation was dependent on the frequency and on the duration of the applied cyclic pressure stimulus: decreased cell proliferation was only observed when these cells were exposed to cyclic pressure at 1.0-Hz (but not at 0.25-Hz) frequency for 1 h (but not for 20 min) daily for 5 days. In contrast, endothelial cells were not responsive to cyclic pressure, whereas fibroblast proliferation increased under similar test conditions. Most important, cyclic pressure affected various osteoblast genes differently: exposure of osteoblasts to cyclic pressure (at 1.0-Hz frequency for 1 h daily) resulted in enhanced transcription and translation of alkaline phosphatase after 5 days; the same mechanical stimulus, however, did not affect osteopontin mRNA expression during the same time periods. These findings provide cellular and molecular level information, which is not only important in elucidating the correlation between mechanical loading and bone homeostasis, but can be useful in development of new technology in skeletal tissue engineering.