Protective effect of prolactin and placental lactogen on NO-induced Nb2 lymphoma cell apoptosis
Protective effect of prolactin and placental lactogen on NO-induced Nb2 lymphoma cell apoptosis
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DOI：
10.1016/s0003-9861(03)00292-3
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发表时间：
2003-08-15
影响因子：
3.9
通讯作者：
Wolfenstein-Todel, C
中科院分区：
文献类型：
--
作者：
Fernández, ML;Iglesias, MM;Wolfenstein-Todel, C
一氧化氮（NO）是一种重要的免疫调节物质。在这里，我们描述的条件下，NO-供体诱导凋亡的Nb 2淋巴瘤细胞，证明细胞活力下降和亚二倍体DNA含量增加，通过流式细胞术测定。琼脂糖凝胶电泳可见典型的凋亡DNA片段化。这种凋亡伴随着caspase-3样酶活性的显著增加。绵羊催乳素（oPRL）和绵羊胎盘催乳素（oPL）对NO供体诱导的细胞凋亡具有保护作用。此外，地塞米松（Dex）诱导的细胞死亡也与caspase-3样活性和oPL具有相同的效力，其保护作用的影响，地塞米松诱导的Nb 2细胞凋亡。(C)2003 Elsevier Science（美国）。All rights reserved.
Nitric oxide (NO) is an important modulator involved in immune regulation. Here, we describe conditions under which NO-donors induce apoptosis on Nb2 lymphoma cells, as evidenced by decreased cell viability and increased hypodiploid DNA content determined by flow cytometry. In addition, DNA fragmentation typical of apoptosis was shown by agarose gel electrophoresis. This apoptosis was accompanied by a significant increase of caspase-3-like enzymatic activity. Both ovine prolactin (oPRL) and ovine placental lactogen (oPL) exerted a protective effect on the NO-donor-induced apoptosis. Furthermore, dexamethasone (Dex)-induced cell death was also associated with caspase-3-like activity and oPL had the same potency as oPRL in its protective effect on Dex-induced apoptosis of Nb2 cells. (C) 2003 Elsevier Science (USA). All rights reserved.