Molecular identification of Tuber magnatum ectomycorrhizae in the field
Molecular identification of Tuber magnatum ectomycorrhizae in the field
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DOI：
10.1016/j.micres.2005.06.003
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发表时间：
2006-01-01
影响因子：
6.7
通讯作者：
Stocchi, V
中科院分区：
文献类型：
--
作者：
Bertini, L;Rossi, I;Stocchi, V
采用分子生物学方法研究了位于意大利中部的Tuber magnatum“truffiere”的块茎外生真菌。利用RFLP-ITS分析、ITS序列测定和特异性探针杂交技术，对335个马铃薯类外生菌根形态类型进行了鉴定，并首次将马铃薯类外生菌根与其他共生种进行了分子鉴定。虽然“truffiere”在调查中产量很好的收获T magnatum子实体，发现的百分比T magnatum ectomycorbia是非常低的（不到4.4%的335根尖分析），而斑点块茎和Tuber rufum的百分比相当高（48.9%和19.0%，分别）。(c)2005年Elsevier GmbH。All rights reserved.
Tuber ectomycorrhizae in a Tuber magnatum "truffiere", located in Central Italy, were studied using molecular methods. Specifically, RFLP-ITS analyses, ITS sequencing and specific probes hybridization were used to identify 335 Tuber-like ectomycorrhizal morphotypes.Molecular identification was possible even when distinct morphological characteristics were lacking.For the first time, T magnatum ectomycorrhizae and other coexisting Tuber species collected in the field were analysed using molecular tools for unambiguous identification. Although the "truffiere" under investigation yields good harvests of T magnatum fruiting bodies, the percentage of T magnatum ectomycorrhizae found was very low (less than 4.4% of the 335 root tips analysed), whereas the percentages of Tuber maculatum and Tuber rufum were considerably higher (48.9% and 19.0%, respectively). (c) 2005 Elsevier GmbH. All rights reserved.