Methylenetetrahydrofolate reductase C677T gene polymorphism and colorectal cancer risk: A case-control study.
Methylenetetrahydrofolate reductase C677T gene polymorphism and colorectal cancer risk: A case-control study.
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亚甲基四氢叶酸还原酶 C677T 基因多态性与结直肠癌风险：病例对照研究。
DOI：
10.3892/ol.2012.740
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发表时间：
2012
期刊：
影响因子：
2.9
通讯作者：
X. Jin
中科院分区：
文献类型：
--
作者：
Guancheng Yin;H. Ming;Xiao Zheng;Y. Xuan;Jianwei Liang;X. Jin
我们设计了一项病例对照研究，以确定血浆同型半胱氨酸（Hcy）水平，并评估亚甲基四氢叶酸还原酶（MTHFR） C677T基因多态性在结直肠癌（CRC）中的潜在作用。在IMx分析仪上使用荧光偏振免疫分析法（FPIA）定量总Hcy。采用实时聚合酶链反应（RT-PCR）分析基因组DNA。结直肠癌组血浆Hcy水平（12.63±3.11 μmol/l）显著高于对照组（10.87±2.42 μmol/l， P<0.05）。MTHFR 677TT基因型在结直肠癌患者中出现的频率明显较高。MTHFR 677TT基因型与CRC风险增加显著相关（优势比为1.671；95%可信区间为1.094-2.553；P=0.018）。本研究提示MTHFR C677T多态性提示CRC易感性并与CRC发病机制相关，提示纯合变异MTHFR C677T多态性是CRC的候选危险因素。
We designed a case-control study to determine the plasma homocysteine (Hcy) level and evaluate the potential role of the methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism in colorectal cancer (CRC). Total Hcy was quantified using the fluorescence polarization immunoassay (FPIA) on the IMx analyzer. Genomic DNA was analyzed by the real-time polymerase chain reaction (RT-PCR). The plasma levels of Hcy in the CRC group (12.63±3.11 μmol/l) were significantly higher compared with those in the control group (10.87±2.42 μmol/l; P<0.05). The frequency of the MTHFR 677TT genotype in CRC patients was markedly high. The MTHFR 677TT genotype was significantly correlated with an increased risk of CRC (odds ratio, 1.671; 95% confidence interval, 1.094-2.553; P=0.018). This study suggests that the MTHFR C677T polymorphism indicates susceptibility to CRC and is correlated with CRC pathogenesis, suggesting that the homozygous variant MTHFR C677T polymorphism is a candidate risk factor for CRC.
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DOI：
10.1073/pnas.94.7.3290
发表时间：
1997-04-01
影响因子：
11.1
作者：
Blount, BC;Mack, MM;Ames, BN
通讯作者：
Ames, BN
影响因子：
7.1
作者：
Kim, YI;Pogribny, IP;Mason, JB
通讯作者：
Mason, JB
叶酸消耗增强结直肠癌发生的机制：统一方案。
DOI：
10.1159/000061830
发表时间：
2000
期刊：
Nestle Nutrition workshop series. Clinical & performance programme.
影响因子：
--
作者：
Mason,JB;Choi,SW
通讯作者：
Choi,SW
影响因子：
4.2
作者：
Choi, SW;Mason, JB
通讯作者：
Mason, JB