Genome-wide transcript analysis of Bradyrhizobium japonicum bacteroids in soybean root nodules
Genome-wide transcript analysis of Bradyrhizobium japonicum bacteroids in soybean root nodules
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DOI：
10.1094/mpmi-20-11-1353
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发表时间：
2007-11-01
影响因子：
3.5
通讯作者：
Hennecke, Hauke
中科院分区：
文献类型：
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作者：
Pessi, Gabriella;Ahrens, Christian H.;Hennecke, Hauke
利用从确定的大豆根瘤中提取的RNA评估了内共生慢生型大豆根瘤菌类细菌的转录组。将结果与B的转录谱进行比较。在需氧或微需氧培养中生长的日本血吸虫细胞。微氧是一种已知的诱发共生相关基因的触发因素。事实上，在接种后第21天，类杆菌中诱导的基因中有三分之一与培养物中通过降低氧浓度上调的基因一致。然而，其他诱导基因可能受到氧限制以外的其他因素的调节。这两组基因为可能发现与共生有关的新功能提供了丰富的来源。在结核发育的不同时间点采集的样本导致了类杆菌中早期和晚期表达的基因的区别。这里应用的实验方法对B也是有用的。japonicum突变体分析。作为一个例子，我们比较了野生型类杆菌的转录组与由RNA聚合酶转录因子σ缺陷突变体形成的类杆菌的转录组（54）。这导致了迄今未被承认的B的收集。日本血吸虫基因可能以σ（54）依赖的方式在植物中转录。
The transcriptome of endosymbiotic Bradyrhizobium japonicum bacteroids was assessed, using RNA extracted from determinate soybean root nodules. Results were compared with the transcript profiles of B. japonicum cells grown in either aerobic or microaerobic culture. Microoxia is a known trigger for the induction of symbiotically relevant genes. In fact, one third of the genes induced in bacteroids at day 21 after inoculation are congruent with those up-regulated in culture by a decreased oxygen concentration. The other induced genes, however, may be regulated by cues other than oxygen limitation. Both groups of genes, provide a rich source for the possible discovery of novel functions related to symbiosis. Samples taken at different timepoints in nodule development have led to the distinction of genes expressed early and late in bacteroids. The experimental approach applied here is also useful for B. japonicum mutant analyses. As an example, we compared the transcriptome of wild-type bacteroids with that of bacteroids formed by a mutant defective in the RNA polymerase transcription factor sigma(54). This led to a collection of hitherto unrecognized B. japonicum genes potentially transcribed in planta in a sigma(54)-dependent manner.