Local differences in vagal afferent innervation of the rat esophagus are reflected by neurochemical differences at the level of the sensory ganglia and by different brainstem projections
Local differences in vagal afferent innervation of the rat esophagus are reflected by neurochemical differences at the level of the sensory ganglia and by different brainstem projections
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DOI：
10.1002/cne.1192
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发表时间：
2001-06-18
影响因子：
2.5
通讯作者：
Neuhuber, WL
中科院分区：
文献类型：
--
作者：
Wank, M;Neuhuber, WL
本研究的目的是进一步表征大鼠食管迷走神经传入纤维，特别是在其最上部，迷走神经感觉神经节中的细胞体，和它们的中央投射。我们应用免疫组织化学的钙视黄蛋白，钙结合蛋白，降钙素基因相关肽（CGRP）;逆行追踪与FluoroGold;和transganglionic追踪与小麦胚芽凝集素辣根过氧化物酶结合神经切除术。迷走神经终末结构在固有肌层的整个食管由calretinin免疫反应神经节内的层状末梢，连接到颈部迷走神经和喉返神经通路。食管最上部的粘膜由一个非常密集的纵向排列的钙视网膜蛋白阳性纤维网支配，该纤维被上级喉神经部分耗尽。在该区域的远端，粘膜几乎没有钙视网膜蛋白免疫反应性迷走神经传入。上颈段食管钙视蛋白阳性粘膜纤维分为四种类型。一种类型，指状末梢，有时也是CGRP的免疫反应性。大约三分之一的迷走神经感觉神经节逆行标记的上颈段食管的细胞体表达CGRP，而三分之二的共表达calretinin和calbindin，但没有CGRP。除孤束核中央亚核外，上颈段食管的迷走神经传入也大量投射到间质亚核。这种额外的投射归因于通过上级喉神经的粘膜传入。本研究提供了一个可能的形态学基础支气管肺反射和厌恶反射后引起的刺激食管。(C)2001 Wiley-Liss，Inc.
The objective of the present study was to characterize further the vagal afferent fibers in the rat esophagus, particularly those in its uppermost part, their cell bodies in vagal sensory ganglia, and their central projections. We applied immunohistochemistry for calretinin, calbindin, and calcitonin gene-related peptide (CGRP); retrograde tracing with FluoroGold; and transganglionic tracing with wheat germ agglutinin-horseradish peroxidase in combination with neurectomies. Vagal terminal structures in the muscularis propria of the whole esophagus consisted of calretinin-immunoreactive intraganglionic laminar endings that were linked to cervical vagal and recurrent laryngeal nerve pathways. The mucosa of the uppermost esophagus was innervated by a very dense net of longitudinally arranged, calretinin-positive fibers that were depleted by section of the superior laryngeal nerve. Distal to this area, the mucosa was virtually devoid of calretinin-immunoreactive vagal afferents. Calretinin-positive mucosal fibers in the upper cervical esophagus were classified into four types. One type, the finger-like endings, was sometimes immunoreactive also for CGRP. About one-third of cell bodies in vagal sensory ganglia retrogradely labeled from the upper cervical esophagus expressed CGRP, whereas two-thirds coexpressed calretinin and calbindin but not CGRP. In addition to the central subnucleus of the nucleus of the solitary tract, vagal afferents from the upper cervical esophagus also projected heavily to the interstitial subnucleus. This additional projection was attributed to mucosal afferents traveling through the superior laryngeal nerve. The present study provides a possible morphological basis for bronchopulmonary and aversive reflexes elicited upon stimulation of the esophagus. (C) 2001 Wiley-Liss, Inc.