Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.
Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.
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DOI：
10.1016/j.xpro.2020.100262
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Fehniger TA
中科院分区：
文献类型：
--
作者：
Wong P;Wagner JA;Berrien-Elliott MM;Schappe T;Fehniger TA
直接杀死病变细胞是 NK 细胞的标志性功能。该协议描述了一种基于流的测定法，用于测量体内激活的小鼠 NK 细胞离体杀死靶细胞的能力。现有公布的用于测定离体 NK 细胞杀伤的方案利用放射性铬释放测定或专为人类 NK 细胞设计。该协议具体详细介绍了使用从体内用聚（I：C）激活的脾细胞富集的原代小鼠NK细胞进行的离体细胞毒性测定。有关该协议的使用和执行的完整详细信息，请参阅。该协议描述了一种基于流的测定法，用于评估小鼠 NK 细胞体外杀伤 协议详细介绍了从小鼠脾细胞中富集 NK 细胞的程序 体内聚（I：C）激活的 NK 细胞用作杀死标记目标的效应器 直接杀死患病细胞是 NK 细胞的标志功能。该协议描述了一种基于流的测定法，用于测量体内激活的小鼠 NK 细胞离体杀死靶细胞的能力。现有公布的用于测定离体 NK 细胞杀伤的方案利用放射性铬释放测定或专为人类 NK 细胞设计。该协议具体详细介绍了使用从体内用聚（I：C）激活的脾细胞富集的原代小鼠NK细胞进行的离体细胞毒性测定。
Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium release assay or were designed for human NK cells. This protocol details specifically an ex vivo cytotoxicity assay using primary murine NK cells enriched from splenocytes that were activated in vivo with poly(I:C). For complete details on the use and execution of this protocol, please refer to. This protocol describes a flow-based assay to assess mouse NK cell killing ex vivo Protocol details the procedure for enriching NK cells from mouse splenocytes In vivo poly(I:C) activated NK cells are used as effectors to kill labeled targets Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium release assay or were designed for human NK cells. This protocol details specifically an ex vivo cytotoxicity assay using primary murine NK cells enriched from splenocytes that were activated in vivo with poly(I:C).
影响因子：
32.4
作者：
Berrien-Elliott, Melissa M.;Sun, Yaping;Fehniger, Todd A.
通讯作者：
Fehniger, Todd A.
影响因子：
8.8
作者：
Wagner, Julia A.;Wong, Pamela;Fehniger, Todd A.
通讯作者：
Fehniger, Todd A.
影响因子：
15.3
作者：
Prager, Isabel;Liesche, Clarissa;Watzl, Carsten
通讯作者：
Watzl, Carsten