Integration-Free Reprogramming of Lamina Propria Progenitor Cells.
Integration-Free Reprogramming of Lamina Propria Progenitor Cells.
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固有层祖细胞的无整合重编程。
DOI：
10.1177/0022034516637579
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发表时间：
2016
影响因子：
7.6
通讯作者：
Howard-Jones RA
中科院分区：
文献类型：
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作者：
Howard-Jones RA
从人体组织中生产诱导多能干细胞（iPSC）用于个性化医疗策略或治疗测试是医学的前沿。因此，确定可通过简单的非侵入性程序轻松获得的用于重编程的细胞来源具有重要的临床意义。通过基因操作的非整合方法将这些细胞重编程为 iPSC 对于再生目的至关重要。在这里，我们展示了接受常规牙科治疗的患者口腔粘膜固有层祖细胞的重编程。利用包含所有 6 个多能性基因（OCT4、SOX2、KLF4、NANOG、LIN28 和 cMYC）的非整合质粒进行重编程。产生的 iPSC 缺乏载体基因的遗传整合，并且具有分化中胚层、外胚层和内胚层谱系的能力，表现出多能性。总之，口腔粘膜固有层祖细胞代表了可以通过最小侵入获得的细胞来源，因为它们可以与常规治疗同时服用。因此，由此产生的免整合 iPSC 在个性化医疗策略中具有巨大的应用潜力。
Producing induced pluripotent stem cells (iPSCs) from human tissue for use in personalized medicine strategies or therapeutic testing is at the forefront of medicine. Therefore, identifying a source of cells to reprogram that is easily accessible via a simple noninvasive procedure is of great clinical importance. Reprogramming these cells to iPSCs through nonintegrating methods for genetic manipulation is paramount for regenerative purposes. Here, we demonstrate reprogramming of oral mucosal lamina propria progenitor cells from patients undergoing routine dental treatment. Reprogramming was performed utilizing nonintegrating plasmids containing all 6 pluripotency genes (OCT4, SOX2, KLF4, NANOG, LIN28, andcMYC). Resulting iPSCs lacked genetic integration of the vector genes and had the ability to differentiate down mesoderm, ectoderm, and endoderm lineages, demonstrating pluripotency. In conclusion, oral mucosal lamina propria progenitor cells represent a source of cells that can be obtained with minimal invasion, as they can be taken concurrently with routine treatments. The resulting integration-free iPSCs therefore have great potential for use in personalized medicine strategies.