Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein.
Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein.
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DOI：
10.3389/fmicb.2014.00744
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发表时间：
2014
影响因子：
5.2
通讯作者：
Connerton IF
中科院分区：
文献类型：
--
作者：
Hooton SP;Connerton IF
空肠弯曲杆菌是引起人类腹泻的世界性疾病。簇状重复间隔回文重复序列(CRISPR)和相关蛋白质允许细菌和古生菌逃避噬菌体和质粒的感染。II型CRISPR系统被发现与编码CRISPR相关的Cas1、Cas2、Cas4或Csn2和Cas9蛋白的基因组合有关。空肠弯曲菌有一个最小的II-C亚型CRISPR系统，包含Cas1、Cas2和Cas9基因，而Cas4明显缺失。Cas4蛋白具有5‘-3’外切酶活性，可产生重组末端以获得间隔区。在这里，我们报道了弯曲菌噬菌体中一个保守的类Cas4蛋白，它在噬菌体和宿主之间创建了一种新的分裂排列，代表了噬菌体/宿主共同进化军备竞赛的新转折。空肠弯曲菌载体状态生命周期中噬菌体和宿主的持续结合为研究该物种的间隔区获得提供了机会。值得注意的是，所有观察到的间隔区序列都来自宿主。我们推测，弯曲菌噬菌体可以利用类Cas4蛋白来激活间隔区的获取，从而利用宿主DNA作为有效的诱饵来诱骗噬菌体DNA。获得自身间隔区并逃脱噬菌体感染的细菌必须克服CRISPR介导的自身免疫，要么通过丧失干扰功能使它们容易受到外来DNA入侵，要么通过耐受基因调控的变化。
Campylobacter jejuni is a worldwide cause of human diarrhoeal disease. Clustered Repetitively Interspaced Palindromic Repeats (CRISPRs) and associated proteins allow Bacteria and Archaea to evade bacteriophage and plasmid infection. Type II CRISPR systems are found in association with combinations of genes encoding the CRISPR-associated Cas1, Cas2, Cas4 or Csn2, and Cas9 proteins. C. jejuni possesses a minimal subtype II-C CRISPR system containing cas1, cas2, and cas9 genes whilst cas4 is notably absent. Cas4 proteins possess 5′-3′ exonuclease activity to create recombinogenic-ends for spacer acquisition. Here we report a conserved Cas4-like protein in Campylobacter bacteriophages that creates a novel split arrangement between the bacteriophage and host that represents a new twist in the bacteriophage/host co-evolutionary arms race. The continuous association of bacteriophage and host in the carrier state life cycle of C. jejuni provided an opportunity to study spacer acquisition in this species. Remarkably all the spacer sequences observed were of host origin. We hypothesize that Campylobacter bacteriophages can use Cas4-like protein to activate spacer acquisition to use host DNA as an effective decoy to bacteriophage DNA. Bacteria that acquire self-spacers and escape phage infection must overcome CRISPR-mediated autoimmunity either by loss of the interference functions leaving them susceptible to foreign DNA incursion or tolerate changes in gene regulation.
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DOI：
10.1038/nrg2749
发表时间：
2010-03
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
3.2
作者：
Deveau, Helene;Barrangou, Rodolphe;Moineau, Sylvain
通讯作者：
Moineau, Sylvain
影响因子：
6.7
作者：
Sampson TR;Weiss DS
通讯作者：
Weiss DS
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y