DIRECT FLUORESCENT LABELING OF CELLS WITH FLUORESCEIN OR RHODAMINE ISOTHIOCYANATE .2. POTENTIAL APPLICATION TO STUDIES OF LYMPHOCYTE MIGRATION AND MATURATION
DIRECT FLUORESCENT LABELING OF CELLS WITH FLUORESCEIN OR RHODAMINE ISOTHIOCYANATE .2. POTENTIAL APPLICATION TO STUDIES OF LYMPHOCYTE MIGRATION AND MATURATION
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DOI：
10.1016/0022-1759(80)90196-9
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发表时间：
1980-01-01
影响因子：
2.2
通讯作者：
WEISSMAN, IL
中科院分区：
文献类型：
--
作者：
BUTCHER, EC;SCOLLAY, RG;WEISSMAN, IL
用荧光素或异硫氰酸四甲基罗丹明直接标记细胞对[小鼠]淋巴细胞迁移的影响进行了检查。定义了标记的体外条件，其不显著影响淋巴细胞的即时或长期活力（体内转移后长达2周），不改变正常淋巴细胞迁移，不影响表面抗原的表达或可检测性，并允许在静脉内注射后数天内直接可视化和用荧光抗体试剂复染。这种方法的潜在应用研究淋巴细胞的迁移和成熟进行了讨论。
The effect of direct cell labeling with fluorescein or tetramethyl rhodamine isothiocyanate on [mouse] lymphocyte migration is examined. In vitro conditions of labeling are defined which do not significantly affect immediate or long term viability of lymphocytes (up to 2 wk after transfer in vivo), do not alter normal lymphocyte migration, do not affect expression or detectability of surface antigens and permit direct visualization and counter-staining with fluorescent antibody reagents for days after i.v. injection. The potential application of this method to studies of lymphocyte migration and maturation is discussed.