SB1 Encoding RING-Like Zinc-Finger Protein Regulates Branch Development as a Transcription Repressor
SB1 Encoding RING-Like Zinc-Finger Protein Regulates Branch Development as a Transcription Repressor
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DOI：
10.1016/j.rsci.2021.04.004
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发表时间：
2021-05
期刊：
影响因子：
4.8
通讯作者：
Zeng Xiaoqin;Zhuang Hui;Q. Cheng;Tang Jun;Yang Fayu;Mingjiang Huang;Wang Ziyi;Zhongcheng Li;Honghui Zhu;Chen Rui;Guanghua He;Yunfeng Li
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作者：
Zeng Xiaoqin;Zhuang Hui;Q. Cheng;Tang Jun;Yang Fayu;Mingjiang Huang;Wang Ziyi;Zhongcheng Li;Honghui Zhu;Chen Rui;Guanghua He;Yunfeng Li
水稻的花序结构，包括分枝的数量和长度，以及小穗的密度，对每穗粒数有很大的影响，是产量构成的关键因素之一。本研究鉴定出5个与水稻分枝发育有关的等位突变体——b1-1/2/3/4/5。在这些突变体中，分支分生组织命运急剧延长，导致从分枝到小穗的过渡延迟，从而增加了每穗的分枝数和小穗数。sb1编码SHI/LRP/SRS家族的一个核环样结构域蛋白，在分支分生组织中强烈表达。蛋白相互作用和染色质免疫沉淀的结果进一步表明，sb1通过与协同抑制复合物相互作用影响靶区组蛋白H3的乙酰化水平，直接抑制dep1、TAW1、moc1和dipa1的表达。因此，我们提出sb1是一个分支分生组织活性的转录抑制因子，通过广泛和负向调节一系列维持分支分生组织命运的基因。
Inflorescence structure of rice, including the number and length of branches, and the density of the spikelet, can greatly affect the number of grains per panicle, which is one of the key factors in yield compositions. Here we identified five allelic mutantssb1-1/2/3/4/5that related to branch development of rice. In these mutants, the branch meristem fate was prolonged sharply, resulting in delay of transition from branches to spikelets, and then increased the numbers of branches and spikelets per panicle.SB1encodes a nuclear RING-like domain protein of SHI/LRP/SRS family and strongly expressed in branch meristems. The results of protein interaction and chromatin immunoprecipitation further suggested thatSB1directly repressed the expression ofDEP1,TAW1,MOC1andIPA1by interacting with a co-repressor complex to affect acetylation level of histone H3 on target regions. Thus, we proposed thatSB1is a transcription repressor of branch meristem activity by widely and negatively regulating a series of genes that maintain branch meristem fate.