A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
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DOI：
10.3791/55591
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发表时间：
2017-07-01
影响因子：
1.2
通讯作者：
Chandy, K. George
中科院分区：
文献类型：
--
作者：
Bose, Tanima;Hou, Aihua;Chandy, K. George
传统上，眼表细胞学是通过抹刀技术和刷子技术等技术进行研究的。这些技术的问题在于，它们可能会引起眼睛表面的创伤性病变，从而导致疤痕、眼睑畸形、角膜缘干细胞缺乏，并且在某些情况下，会给受试者带来极大的不适。为了避免这些临床问题，印迹细胞学 (IC) 被开发来诊断干眼病以及后来的肿瘤、特应性疾病、春季角结膜炎和干燥性角结膜炎。通常，临床医生手动将滤纸切割成所需的形状并将其应用到眼表面。在这里，我们描述如何使用市售医疗设备执行 IC。这里解释了该技术，然后通过流式细胞术进行免疫表型分析。该技术需要较少的手动操作，并且对眼表造成的伤害较小。
Traditionally, ocular surface cytology is studied with techniques such as spatula technology and brush technology. The problem with these techniques is that they may induce traumatic lesions on the surface of the eye, which can progress to scarring, eyelid deformity, limbal stem cell deficiency and in some cases, cause great discomfort to the subject. To avoid these clinical problems, impression cytology (IC) was developed to diagnose dry eye disease and later neoplasia, atopic disease, vernal keratoconjunctivitis and keratoconjunctivitis sicca. Typically, clinicians manually cut filter papers into required shapes and apply these to the ocular surface. Here, we describe how to perform IC using a commercially available medical device. This technique is explained here followed by immunophenotyping by flow cytometry. This technique requires less manual handling and causes less injury to the ocular surface.