1H and 13C NMR assignments and molecular modelling of a minor groove DNA-binding peptide from the HMG-I protein.
1H and 13C NMR assignments and molecular modelling of a minor groove DNA-binding peptide from the HMG-I protein.
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HMG-I 蛋白小沟 DNA 结合肽的 1H 和 13C NMR 归属和分子建模。
DOI：
10.1111/j.1399-3011.1995.tb01319.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Reeves,R
中科院分区：
文献类型：
--
作者：
Evans,JN;Zajicek,J;Nissen,MS;Munske,G;Smith,V;Reeves,R
高迁移率族（HMG）染色质蛋白的HMG-I亚家族由DNA结合蛋白组成，其在体外和体内均优先结合富含A·T-序列的片段。最近，已经提出HMG-I家族的成员通过11个氨基酸的肽结合结构域（BD）体外结合A·T-DNA的狭窄小沟，由于其预测的结构，其被称为“A·T-钩基序”[Reeves，R. & Nissen，M.（1990）J.Biol.Chem.265，8573-8582]，并且看起来是新月形的。合成了具有13个氨基酸残基的BD肽，并通过质子和碳-13核磁共振（NMR）光谱进行了检查。该肽含有4个脯氨酸残基，NOES和13 C化学位移的基础上，发现存在全反式构象。基于该结果的分子建模提供了溶液中转弯构象之间动态平衡的证据，其中最常见的可能是基于酰胺交换数据的S形构象。© Munksgaard 1995.
The HMG‐I subfamily of high mobility group (HMG) chromatin proteins consists of DNA‐binding proteins that preferentially bind to stretches of A·T‐rich sequence bothin vitroandin vivo.Recently, members of the HMG‐I family have been suggested to bindin vitroto the narrow minor groove of A·T‐DNA by means of an 11 amino acid peptide binding domain (BD) which, because of its predicted structure, is called the‘A·T‐hook motif [Reeves, R. & Nissen, M. (1990)J. Biol. Chem.265, 8573–8582], and would appear to be crescent‐shaped. A BD peptide with 13 amino‐acid residues was synthesized and examined by proton and carbon‐13 nuclear magnetic resonance (NMR) spectroscopy. The peptide contains four proline residues, and on the basis of NOES and13C chemical shifts was found to exist in an all‐transconformation. Molecular modelling based on this result provides evidence for a dynamic equilibrium between turn‐like conformations in solution, the most populated of which is likely to be an S‐shaped conformer, on the basis of amide exchange data. © Munksgaard 1995.