Isolation and Characterization of Side Population Cells in the Postpartum Murine Endometrium
Isolation and Characterization of Side Population Cells in the Postpartum Murine Endometrium
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DOI：
10.1177/1933719110369180
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发表时间：
2010-07-01
影响因子：
2.9
通讯作者：
Liu, Jia-Yin
中科院分区：
文献类型：
--
作者：
Hu, Fei-Fei;Xu, Jing;Liu, Jia-Yin
子宫内膜是一个高度活跃的器官，在生命周期中定期重塑。以前的研究表明，在这种组织中存在成体干细胞或祖细胞群。在这项研究中，侧群（SP）细胞分离自产后小鼠子宫的子宫内膜，但不从子宫的子宫内膜进行正常的发情周期。表型分析显示SP细胞造血、内皮和间充质干细胞（MSC）标记均阴性，但不同程度地表达干细胞抗原1（Sca-1）和c-kit。侧群细胞是具有集落形成能力的子宫内膜干/祖细胞的异质群体。他们被发现驻留在静止的基质，但不是在腔上皮。这些数据表明，与其他组织和器官一样，小鼠子宫内膜也含有SP细胞。它们在子宫内膜再生中的具体作用值得进一步研究。
Endometrium is a highly active organ that is periodically remodeled during the life span. Previous studies have indicated the presence of an adult stem or progenitor cell population in this tissue. In this study, side population (SP) cells were isolated from the endometrium of postpartum murine uterus but not from the endometrium of a uterus undergoing a normal estrus cycle. Phenotype analysis showed that SP cells were negative for hematopoietic, endothelial, and mesenchymal stem cell (MSC) markers, but they expressed stem cell antigen 1 (Sca-1) and c-kit at various degrees. Side population cell is a heterogeneous population of endometrial stem/progenitor cells that have colony-forming capacity. They were found to reside in quiescence in the stroma but not in the luminal epithelium. These data suggest that, like other tissues and organs, the murine endometrium also contains SP cells. Their specific role in the regeneration of the endometrium warrants further study.