Data mining the arabidopsis genome reveals fifteen 14-3-3 genes. Expression is demonstrated for two out of five novel genes
Data mining the arabidopsis genome reveals fifteen 14-3-3 genes. Expression is demonstrated for two out of five novel genes
复制标题
DOI：
10.1104/pp.127.1.142
复制
发表时间：
2001-09-01
期刊：
影响因子：
7.4
通讯作者：
Sommarin, M
中科院分区：
文献类型：
--
作者：
Rosenquist, M;Alsterfjord, M;Sommarin, M
在植物中，14-3-3蛋白是初级代谢和膜运输的关键调节剂。尽管目前的教条认为14-3-3同种型对靶蛋白的特异性不高，但最近的数据表明其特异性可能很高。因此，在模式植物拟南芥中鉴定和表征所有14-3-3（GF 14）同种型是重要的。利用拟南芥信息资源中的信息，我们发现了三个新的GF 14基因。这三种基因和另外两种新的GF 14基因的潜在表达（Rosenquist et al.，2000），在叶、根和花中使用逆转录酶-聚合酶链反应和cDNA文库聚合酶链反应筛选进行检查。在正常生长条件下，发现其中两个基因被转录。这些基因被命名为grf 11和grf 12，相应的新的14-3-3亚型分别被命名为GF 14 omicron和GF 14 iota。编码GF 14 α的基因在叶、根和花中表达，而编码GF 14 β的基因仅在花中表达。基因结构和GF 14基因家族所有成员之间的关系是从拟南芥信息资源中获得的数据推断出来的。这些数据清楚地支持了真双子叶植物从单子叶植物分化时存在两个14-3-3基因的理论。在拟南芥中总共有15个14-3-3基因（grfs 1-15），其中12个（grfs 1-12）现在已经被证明是表达的。
In plants, 14-3-3 proteins are key regulators of primary metabolism and membrane transport. Although the current dogma states that 14-3-3 isoforms are not very specific with regard to target proteins, recent data suggest that the specificity may be high. Therefore, identification and characterization of all 14-3-3 (GF14) isoforms in the model plant Arabidopsis are important. Using the information now available from The Arabidopsis Information Resource, we found three new GF14 genes. The potential expression of these three genes, and of two additional novel GF14 genes (Rosenquist et al., 2000), in leaves, roots, and flowers was examined using reverse transcriptase-polymerase chain reaction and cDNA library polymerase chain reaction screening. Under normal growth conditions, two of these genes were found to be transcribed. These genes were named grf11 and grf12, and the corresponding new 14-3-3 isoforms were named GF14omicron and GF14iota, respectively. The gene coding for GF14omicron was expressed in leaves, roots, and flowers, whereas the gene coding for GF14iota was only expressed in flowers. Gene structures and relationships between all members of the GF14 gene family were deduced from data available through The Arabidopsis Information Resource. The data clearly support the theory that two 14-3-3 genes were present when eudicotyledons diverged from monocotyledons. In total, there are 15 14-3-3 genes (grfs 1-15) in Arabidopsis, of which 12 (grfs 1-12) now have been shown to be expressed.