Intrahippocampal grafts containing cholinergic and serotonergic fetal neurons ameliorate spatial reference but not working memory in rats with fimbria-fornix/cingular bundle lesions
Intrahippocampal grafts containing cholinergic and serotonergic fetal neurons ameliorate spatial reference but not working memory in rats with fimbria-fornix/cingular bundle lesions
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DOI：
10.1016/s0361-9230(99)00058-1
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发表时间：
1999-07-01
影响因子：
3.8
通讯作者：
Cassel, JC
中科院分区：
文献类型：
--
作者：
Balse, E;Lazarus, C;Cassel, JC
三个月大的Long-Evans雌性大鼠持续抽吸病变的背侧隔海马通路，2周后，接受海马内悬浮移植物含有细胞从中脑缝，细胞从内侧隔和斜角带的布罗卡，或两者的混合物。仅病变和假手术大鼠用作对照。所有大鼠在损伤手术后1周、3和5个月进行自发活动测试，在5至9个月进行放射状迷宫中的空间工作记忆测试，并在损伤后9个月进行水箱中的参考和工作记忆测试。在完成行为测试后测定海马中乙酰胆碱、去甲肾上腺素和5-羟色胺的浓度。与假手术大鼠相比，所有病变大鼠，无论是否移植，表现出增加的自主活动水平，并作出更多的错误，在径向迷宫。根据要求完整参考记忆能力的协议，在水箱测试的探针试验（前30秒）中，受损大鼠也受损。虽然隔或中缝移植的大鼠也受损，与共同移植的大鼠表现出与假手术大鼠没有显着差异。由于方案要求工作记忆能力完好无损，所有受损大鼠，无论是否移植，在水箱测试中都受到损害。在损伤只大鼠的背海马，乙酰胆碱和5-羟色胺的浓度显着降低。在隔移植或cc移植的大鼠中，乙酰胆碱的浓度接近正常，在中缝移植或共同移植的大鼠中5-羟色胺的浓度也接近正常。这些结果证实了先前的研究结果，即共同移植物使单一移植物的神经化学性质相结合。水箱试验的数据表明，胆碱能和海马神经的胚胎细胞移植可能会导致部分恢复的空间参考，但不是工作记忆能力的大鼠。(C)1999 Elsevier Science Inc.
Three-month-old Long-Evans female rats sustained aspirative lesions of the dorsal septohippocampal pathways and, 2 weeks later; received intrahippocampal suspension grafts containing cells from the mesencephalic raphe, cells from the medial septum and the diagonal band of Broca, or a mixture of both. Lesion-only and sham-operated rats were used as controls. Ail rats were tested for locomotor activity 1 week, 3 and 5 months after lesion surgery, for spatial working memory in a radial maze from 5 to 9 months, and for reference and working memory in a water tank during the 9th month after lesioning. Determination of hippocampal concentration of acetylcholine, noradrenaline, and serotonin was made after completion of behavioral testing. Compared to sham-operated rats, all rats with lesions, whether grafted or not, exhibited increased levels of locomotor activity and made more errors in the radial maze. The lesioned rats were also impaired in the probe trial (30 first seconds) of the water-tank test made according to a protocol requiring intact reference memory capabilities. While rats with septal or raphe grafts were also impaired, the rats with co-grafts showed performances not significantly different from those of sham-operated rats. With a protocol requiring intact working memory capabilities, all lesioned rats, whether grafted or not, were impaired in the water-tank test. In the dorsal hippocampus of lesion-only rats, the concentration of acetylcholine and serotonin was significantly reduced. In rats with septal grafts or cc-grafts, the concentration of acetylcholine was close to normal, as was that of serotonin in rats with raphe grafts or co-grafts. These results confirm previous findings showing that co-grafts enabled the neurochemical properties of single grafts to be combined. Data from the water-tank test suggest that cholinergic and serotonergic hippocampal reinnervations by fetal cell grafts may induce partial recovery of spatial reference, but not working memory capabilities in rats. (C) 1999 Elsevier Science Inc.