The role of IS26 in evolution of a derivative of the virulence plasmid of Salmonella enterica serovar Enteritidis which confers multiple drug resistance
The role of IS26 in evolution of a derivative of the virulence plasmid of Salmonella enterica serovar Enteritidis which confers multiple drug resistance
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DOI：
10.1016/j.meegid.2016.09.008
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发表时间：
2016-11-01
影响因子：
3.2
通讯作者：
Rosario Rodicio, M.
中科院分区：
文献类型：
--
作者：
Garcia, Vanesa;Garcia, Patricia;Rosario Rodicio, M.
pUO-SeVR 1是pSEV的抗性衍生物，pSEV是肠道沙门氏菌肠道血清型特异性的毒力质粒。它首先在涉及胃肠炎的西班牙分离株中检测到，但密切相关的质粒在源自非洲的高度侵袭性分离株中广泛存在。根据其核苷酸序列，pUO SeVR 1由110，982 bp组成，GC含量为53.2%。它赋予对多种抗菌剂的抗性，并含有丰富的移动的遗传元件，包括在质粒进化中起主要作用的14个IS 26拷贝。还检测到IS 4321的完整拷贝和许多其他转座因子（Tn 2、Tn 21、Tn 1721和Tn 5393）的残余。大部分pSEV序列在pUO-SeVR 1中是保守的，但发生了小的缺失、主要倒位和两个外源区域（38.7和13.6 kb，其中抗性基因成簇）的插入。IncL/M、IncN和IncQ基团的质粒酶可能有助于抗性区域的组装。(C)© 2016 Elsevier B. V.版权所有。
pUO-SeVR1 is a resistance derivative of pSEV, the virulence plasmid specific of Salmonella enterica serovar Enteritidis. It was first detected in a Spanish isolate involved in gastroenteritis, but closely related plasmids are widespread in highly invasive isolates originating from Africa. According to its nucleotide sequence, pUO-SeVR1 consists of 110,982 bp with a GC content of 53.2%. It confers resistance to multiple antimicrobial agents and contains a wealth of mobile genetic elements, including 14 copies of IS26 which have played a major role in the evolution of the plasmid. An intact copy of IS4321 and remnants of many other transposable elements (Tn2, Tn21, Tn1721 and Tn5393) are also detected. Most of the pSEV sequence is conserved in pUO-SeVR1 but small deletions, major inversions and the insertion of two foreign regions (of 38.7 and 13.6 kb, where the resistance genes are clustered), have occurred. Plasmids of the IncL/M, IncN and IncQ groups could have contributed to the assembly of the resistance regions. (C) 2016 Elsevier B.V. All rights reserved.