Fast Standardized Therapeutic-Efficacy Assay for Drug Discovery against Tuberculosis
Fast Standardized Therapeutic-Efficacy Assay for Drug Discovery against Tuberculosis
复制标题
DOI：
10.1128/aac.01423-09
复制
发表时间：
2010-05-01
影响因子：
4.9
通讯作者：
Angulo-Barturen, Inigo
中科院分区：
文献类型：
--
作者：
Rullas, Joaquin;Ignacio Garcia, Juan;Angulo-Barturen, Inigo
小鼠结核分枝杆菌感染模型是药物发现的重要工具。在这里，我们描述了一种快速标准化的9天急性试验，旨在测量药物对免疫能力强的小鼠肺部生长的结核分枝杆菌的疗效。该分析重复性高，允许良好的吞吐量，并通过使用异烟肼、利福平、乙胺丁醇、吡津酰胺、利奈唑胺和莫西沙星进行药物先导优化验证。
Murine models of Mycobacterium tuberculosis infection are essential tools in drug discovery. Here we describe a fast standardized 9-day acute assay intended to measure the efficacy of drugs against M. tuberculosis growing in the lungs of immunocompetent mice. This assay is highly reproducible, allows good throughput, and was validated for drug lead optimization using isoniazid, rifampin, ethambutol, pyrazinamide, linezolid, and moxifloxacin.