Cross-linking analysis reveals the putative dimer structure of the cyanobacterial BLUF photoreceptor PixD
Cross-linking analysis reveals the putative dimer structure of the cyanobacterial BLUF photoreceptor PixD
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交联分析揭示了蓝藻 BLUF 光感受器 PixD 的假定二聚体结构
DOI：
10.1016/j.febslet.2015.05.019
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Ren;S.;Sugimoto;Y.;Kobayashi;T. and Masuda;S.
PixD是蓝细菌集胞藻（Synechocystissp）中使用黄素（BLUF）型蓝光光受体控制趋光性的蓝光。PCC6803。PixD的晶体结构显示十聚体，尽管在溶液中二聚体和十聚体之间保持平衡。因为这两种构象的比例会因光照而改变，所以平衡状态决定了光敏性。然而，没有关于PixD二聚体的结构信息。在这里，我们报告的二聚体的对接模拟，诱变和基于质谱的交联分析的基础上预测的结构。结果表明PixD C-末端对于二聚体偏好和光敏性的重要性。
PixD is a blue light using flavin (BLUF)-type blue-light photoreceptor controlling phototaxis in the cyanobacteriumSynechocystissp. PCC6803. The crystal structure of PixD shows a decamer, although in solution an equilibrium is maintained between the dimer and decamer. Because the ratio of these two conformers is altered by illumination, the equilibrium state determines photosensitivity. However, no structural information is available for the PixD dimer. Here, we report a predicted structure for the dimer based on docking simulation, mutagenesis, and mass spectrometry-based cross-linking analyses. The results indicate the importance of the PixD C-terminus for dimer preference and photosensitivity.