Identification of a novel endophytic fungus from Huperzia serrata which produces huperzine A
Identification of a novel endophytic fungus from Huperzia serrata which produces huperzine A
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产石杉碱甲的蛇足石杉中新型内生真菌的鉴定
DOI：
10.1007/s11274-014-1737-6
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发表时间：
2014-12-01
影响因子：
4.1
通讯作者：
Wang, Mo
中科院分区：
文献类型：
--
作者：
Shu, Shaohua;Zhao, Xinmei;Wang, Mo
石杉碱A是从锯齿石杉中分离出来的，因其毒性低、有效期长而被用于治疗阿尔茨海默病。serrata来源的减少意味着天然石杉碱A不能满足临床治疗的需要。本研究从锯齿木牙内生真菌中分离得到>200株，并采用高效液相色谱法进行筛选。采用液相色谱-串联质谱法对菌株ES026产石杉碱A进行了鉴定和定量，石杉碱A的产率为1 μ g/g干菌丝。菌株ES026通过形态、特异引物聚合酶链反应和rDNA内转录间隔序列鉴定为炭疽菌gloeosporioides。ES026有利于培育石杉碱A高产栽培菌株，同时该菌株适合石杉碱A的生物合成研究。
Huperzine A is isolated from Huperzia serrata and is used for treatment of Alzheimer's disease, due to its low toxicity and long effective period. The decrease in H. serrata sources means that natural huperzine A cannot meet the needs of clinical therapy. In this study, > 200 endophytic fungal strains were isolated from H. serrata, and screened using high-performance liquid chromatography. Strain ES026 produced huperzine A. Production was identified and quantified by liquid chromatography-tandem mass spectrometry, and the yield of huperzine A was 1 mu g/g dried mycelium. ES026 strain was identified as Colletotrichum gloeosporioides by morphology, polymerase chain reaction with species-specific primers and rDNA internal transcribed spacer sequence. ES026 contributes to the breeding of cultivated strains with high yield of huperzine A. Meanwhile, the strain was suitable for the study of biosynthesis of huperzine A.