Atriopeptin II relaxes and elevates cGMP in bovine pulmonary artery but not vein.
Atriopeptin II relaxes and elevates cGMP in bovine pulmonary artery but not vein.
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Atriopeptin II 可放松并升高牛肺动脉而非静脉中的 cGMP。
DOI：
10.1152/jappl.1986.60.4.1128
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Kadowitz,PJ
中科院分区：
文献类型：
--
作者：
Ignarro,LJ;Wood,KS;Harbison,RG;Kadowitz,PJ
Atriopeptin II 是一种心房钠尿因子的 23 个氨基酸合成肽片段，可引起牛肺内动脉分离的预收缩环的内皮依赖性松弛，并伴随着鸟苷 3',5'-环单磷酸 (cGMP) 的积累，但不会引起腺苷 3',5'-环单磷酸的积累。相反，无论是否存在内皮细胞，肺内静脉环均不受心肽II的影响。亚甲蓝（可溶性鸟苷酸环化酶抑制剂）可分别消除对乙酰胆碱和三硝酸甘油的反应的内皮依赖性和独立性动脉舒张和 cGMP 积累，但亚甲蓝未能改变对心肽素 II 的这些反应。同样，心得安、吲哚美辛或阿托品也不会改变心肽素 II 的作用。这些结果表明，心肽素 II 引起的舒张可能对动脉平滑肌受体具有选择性，不需要内皮细胞，并且不涉及可溶形式的鸟苷酸环化酶，尽管刺激了 cGMP 积累。
Atriopeptin II, a 23-amino acid synthetic peptide fragment of atrial natriuretic factor, caused an endothelium-independent relaxation of isolated precontracted rings of bovine intrapulmonary artery that was accompanied by the concomitant accumulation of guanosine 3′,5′-cyclic monophosphate (cGMP) but not adenosine 3′,5′-cyclic monophosphate. In contrast, rings of intrapulmonary vein were unaffected by atriopeptin II whether or not endothelium was present. Whereas methylene blue, an inhibitor of soluble guanylate cyclase, abolishes endothelium-dependent and independent arterial relaxation and cGMP accumulation in response to acetylcholine and glyceryl trinitrate, respectively, methylene blue failed to alter these responses to atriopeptin II. Similarly, the effects of atriopeptin II were unaltered by propranolol, indomethacin, or atropine. These results indicate that relaxation elicited by atriopeptin II may be selective for arterial smooth muscle receptors, does not require endothelial cells, and does not involve the soluble form of guanylate cyclase, although cGMP accumulation is stimulated.