The polysaccharides from the fruits of Lycium barbarum L. confer anti-diabetic effect by regulating gut microbiota and intestinal barrier
The polysaccharides from the fruits of Lycium barbarum L. confer anti-diabetic effect by regulating gut microbiota and intestinal barrier
复制标题
枸杞果实中的多糖通过调节肠道菌群和肠道屏障发挥抗糖尿病作用
DOI：
10.1016/j.carbpol.2022.119626
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发表时间：
2022-05-19
影响因子：
11.2
通讯作者：
Cao, Youlong
中科院分区：
文献类型：
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作者：
Zhou, Wangting;Yang, Tingting;Cao, Youlong
枸杞子多糖抗糖尿病作用及其机制的研究。通过高脂饮食/链脲佐菌素诱导的糖尿病小鼠模型研究了LBPs。口服LBP（200 mg/kg/d）6周后，糖尿病小鼠的空腹血糖（下降13.51%）、糖化血红蛋白水平和β细胞功能均得到改善，同时肠道细菌群落中一种Allobaculum属的细菌数量增加了3.3倍。粪便微生物群移植和抗生素治疗实验证实了LBP介导的肠道微生物群参与糖尿病管理的血糖控制。此外，LBPs干预通过在体内（分析糖尿病小鼠的肠道通透性）和体外（使用结肠样Caco-2/RAW264.7细胞共培养炎症模型）上调闭合小带1的表达来保护肠屏障功能。总的来说，我们的研究表明LBP可以通过改变肠道微生物群和肠道屏障来赋予抗糖尿病作用。
The antidiabetic effect and potential mechanisms of the polysaccharides from the fruits of Lycium barbarum L. (LBPs) by the mouse model of high-fat diet/streptozotocin-induced diabetes were investigated. Six-week oral administration of LBPs (200 mg/kg/day) resulted in improvement in the levels of fasting blood glucose (13.51% decrease) and glycated hemoglobin and beta-cell function in diabetic mice, and simultaneously induced a 3.3-fold increment in one taxon belonging to genus Allobaculum in gut bacterial community. The experiments of fecal microbiota transplantation and antibiotics treatment confirmed that the LBPs-mediated gut microbiota participated in the glycemic control of the diabetes management. Moreover, LBPs intervention guarded the intestinal barrier function via upregulating the expression of zonula occludens 1 both in vivo (analyzing the gut permeability in diabetic mice) and in vitro (using intestinal-like Caco-2/RAW264.7 cells co-culture inflammation model). Collectively, our study showed that LBPs could confer anti-diabetic effect through modifying gut microbiota and intestinal barrier.