Protective effect of 20-hydroxyeicosatetraenoic acid (20-HETE) on glomerular protein permeability barrier
Protective effect of 20-hydroxyeicosatetraenoic acid (20-HETE) on glomerular protein permeability barrier
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DOI：
10.1111/j.1523-1755.2005.00065.x
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发表时间：
2005-01-01
影响因子：
19.6
通讯作者：
Sharma, M
中科院分区：
文献类型：
--
作者：
McCarthy, ET;Sharma, R;Sharma, M
Background. Proteinuria is a significant problem in medicine today, although glomerular events underlying it are unknown. Products of cytochrome P450 (CYP450) pathway of arachidonic acid metabolism are increasingly recognized as playing major roles in renal function. We used in vitro albumin permeability (P-alb) as a measure of injury and puromycin aminonucleoside (PAN) as an injurious agent to test the hypothesis that 20-hydroxyeicosatetraenoic acid (20-HETE) protects the glomerular filtration barrier from increased P-alb.Methods. We determined P-alb in the following experimental groups: (1) isolated rat glomeruli incubated with PAN( 5 mug/mL) for 5, 15, 30 or 60 minutes; (2) isolated glomeruli preincubated with 20-HETE (1.0 nmol/L to 100 nmol/L) for 15 minutes followed by additional incubation with PAN (5 mug/mL) for 15 minutes; (3) isolated glomeruli from rats treated with the CYP450 4A inducer clofibrate, and incubated with PAN (5 mug/mL) for 15 minutes; and (4) appropriate controls for each group. CYP450 4A levels were measured in glomeruli isolated from rats treated with clofibrate or vehicle.Results. PAN increased P-alb of isolated glomeruli as early as 5 minutes (P-alb 0.33+/-0.21, P
