The fibroblast growth factor receptors, FGFR-1 and FGFR-2, mediate two independent signalling pathways in human retinal pigment epithelial cells.
The fibroblast growth factor receptors, FGFR-1 and FGFR-2, mediate two independent signalling pathways in human retinal pigment epithelial cells.
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DOI：
10.1016/j.bbrc.2005.09.028
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发表时间：
2005-11
影响因子：
3.1
通讯作者：
R. Rosenthal;G. Malek;N. Salomon;Mortiz Peill-Meininghaus;Linn Coeppicus;Hendrik Wohlleben;S. Wimmers;Catherine Bowes Rickman;O. Strauß
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文献类型：
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作者：
R. Rosenthal;G. Malek;N. Salomon;Mortiz Peill-Meininghaus;Linn Coeppicus;Hendrik Wohlleben;S. Wimmers;Catherine Bowes Rickman;O. Strauß
To examine the effects and potential implications for the expression of the two basic fibroblast growth factor (bFGF) receptors, FGFR-1 and FGFR-2, in retinal pigment epithelial (RPE) cells, bFGF-dependent changes in gene expression and RPE cell function were studied. bFGF increased L-type Ca2+channel activity of RPE cells, which in turn resulted in an increase of vascular endothelial growth factor A (VEGF-A) secretion from RPE cells. Also, both bFGF and direct stimulation of L-type Ca2+channels by BayK8644 increased the expression of c-fos in RPE cells, to the same extent. bFGF-induced-c-fos expression was reduced by inhibition of FGFR-1, but not by L-type Ca2+channel inhibition, demonstrating that stimulation of FGFR-1 results in a Ca2+channel-independent change of gene expression. In contrast, stimulation of FGFR-2 results in a Ca2+channel-dependent stimulation of VEGF secretion. Furthermore, immunohistological investigation of neovascular tissues obtained from patients with age-related macular degeneration (AMD) revealed FGFR-1 and FGFR-2 expression in the RPE of the diseased tissue. Our findings support the hypothesis that there are two different FGFR-1- and FGFR-2-dependent pathways that modulate the role of bFGF in induction of neovascularisation in AMD.
