Nonsurgical periodontal treatment reduced aortic inflammation in ApoE(-/-) mice with periodontitis.
Nonsurgical periodontal treatment reduced aortic inflammation in ApoE(-/-) mice with periodontitis.
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DOI：
10.1186/s40064-016-2637-z
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Yan F
中科院分区：
文献类型：
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作者：
Cui D;Li H;Lei L;Chen C;Yan F
虽然牙周感染与动脉粥样硬化病变之间的相关性已经得到了很好的认识，但非手术牙周治疗是否以及如何改善血管炎症还没有明确的研究。载脂蛋白E−/−(apoE−/−)小鼠32只，随机分为4组：(1)对照组：不给药，空白对照组；(2) ligg组：结扎性牙周炎组；(3) ligo - n组：第7天拔除结扎；(4) ligi -SRP组：第7天拔除结扎物，第9天进行刮治和根刨（SRP）。第30天对所有动物实施安乐死。在微计算机断层扫描下评估牙槽骨丢失（ABL）。检测全身炎症状态和血浆脂质含量。通过免疫组织学和实时荧光定量PCR评估几种血管炎症替代标志物的表达。在apoE−/−小鼠中，NSPT降低ABL，改善血脂，并通过降低血浆白细胞介素-6 （IL-6）水平抑制全身炎症；此外，NSPT处理小鼠动脉壁炎症减轻，血管细胞粘附分子-1表达减少，巨噬细胞粘附减少；此外，NSPT改善了主动脉壁弹性纤维断裂紊乱，从而保持了主动脉的弹性。结扎引起的牙周炎可引起血管壁的炎症反应，NSPT通过减少全身炎症和改善脂质水平对关节壁动脉粥样硬化的早期过程有有益的作用。
Although the correlation between periodontal infection and atherosclerotic lesions has been well recognized, whether and how the nonsurgical periodontal treatment (NSPT) can improve the vascular inflammation has not been investigated clearly. Thirty-two apolipoprotein E−/− (apoE−/−) mice were randomly divided into four groups: (1) Con group: no treatment, blank control group; (2) Lig group: ligature-induced-periodontitis group; (3) Lig-N group: ligatures were removed on the 7th day; (4) Lig-SRP group: ligatures were removed on the 7th day, and scaling and root planing (SRP) were performed on the 9th day. All the animals were euthanized on the 30th day. Alveolar bone loss (ABL) was assessed under microcomputed tomography. Systemic inflammatory status and lipid contents in the plasma were detected. Expression of several surrogate markers for vascular inflammation was evaluated by immunohistology and quantitative real time PCR. NSPT reduced ABL, improved lipid profile, and inhibited systemic inflammation with reduced plasma interleukin-6 (IL-6) level in apoE−/− mice; in addition, reduced inflammation in arterial wall was observed in NSPT treated mice, showing less vascular cell adhesion molecule-1 expression and less macrophage adhesion; furthermore, NSPT improved elastic fiber fragmentation disorder in the aortic wall, thus preserved elasticity of aortic artery. Ligature-induced periodontitis can lead to inflammatory response in the vascular wall and NSPT has beneficial effect on the early stage of atherosclerosis process in the articular wall by reducing systemic inflammation and improving lipid profile.