Electrophoretic behavior of d(GGAAAAAAGG)n, d(CCAAAAAACC)n, and (CCAAAAAAGG)n and implications for a DNA bending model.
Electrophoretic behavior of d(GGAAAAAAGG)n, d(CCAAAAAACC)n, and (CCAAAAAAGG)n and implications for a DNA bending model.
复制标题
d(GGAAAAAAGG)n、d(CCAAAAAACC)n 和 (CCAAAAAAGG)n 的电泳行为以及对 DNA 弯曲模型的影响。
DOI：
--
复制
发表时间：
1990
影响因子：
14.9
通讯作者：
A. V. Ulyanov
中科院分区：
文献类型：
--
作者：
R. Abagyan;V. Mironov;B. Chernov;V. P. Chuprina;A. V. Ulyanov
从化学合成的寡核苷酸制备双链多聚体（C2 A6 C2）n、（C2 A6 G2）n和（G2 A6 G2）n，以研究An区侧翼序列对DNA曲率的影响。所有这些双链体，包括聚嘌呤、聚嘧啶1，在聚丙烯酰胺凝胶中表现出强烈的阻滞，这表明明显的DNA弯曲。先前已经提出，在与寡核苷酸（A）束的边界处的弯曲中，应区分两种类型：5 ′-弯曲和3 ′-弯曲（Koo等人，1986）这种区别是从两种特别设计的序列的不同相对迁移率推导出来的，这两种序列分别具有定相的5 '-弯曲和3'-弯曲。我们的数据表明，在A6区的5'和3'边界处的核苷酸取代导致相对迁移率的可比变化。因此，对于B-B'弯曲，重要的是不仅要考虑它们是在寡核苷酸（dA）区的5'端还是3'端，而且要考虑在该区的边界处的特定序列。
Double stranded multimers (C2A6C2)n, (C2A6G2)n and (G2A6G2)n were prepared from chemically synthesized oligonucleotides to study the influence of sequences flanking the An tract on the curvature of DNA. All these duplexes, including polypurine.polypyrimidine one, exhibit strong retardation in polyacrylamide gel which is indicative of pronounced DNA curvature. It has been proposed previously that among the bends at the boundary with the oligo(A) tract two types should be distinguished: 5'-bends and 3'-bends (Koo et al., 1986) This distinction was deduced from different relative mobilities of two specially designed sequences having phased 5'-bends and 3'-bends, respectively. Our data indicate that the substitutions of nucleotides at both 5' and 3' boundaries of A6 tract result in comparable changes in relative mobility. Therefore, for B-B' bends it is important to take into account not only whether they are at the 5' or 3' end of an oligo(dA) tract, but also the particular sequences at the boundaries of this tract.