Microfluidic and optical systems for the on-demand generation and manipulation of single femtoliter-volume aqueous droplets
Microfluidic and optical systems for the on-demand generation and manipulation of single femtoliter-volume aqueous droplets
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DOI：
10.1021/ac060748l
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发表时间：
2006-09-15
影响因子：
7.4
通讯作者：
Chiu, Daniel T.
中科院分区：
文献类型：
--
作者：
Lorenz, Robert M.;Edgar, J. Scott;Chiu, Daniel T.
本文介绍了一种流体和光学平台的产生和操作的单个飞升体积的水液滴。使用赋予环境流动稳定性的微流体腔室按需产生单个液滴。实施光学涡旋阱来操纵和传输所产生的液滴，所述液滴具有比液滴浸没在其中的不混溶介质更低的折射率，因此不能使用常规的光学镊子来捕获。我们还证明了收缩和增加所产生的液滴的折射率的能力，从而允许其使用光学镊子与另一个液滴容易融合。为了说明该平台的多功能性，我们在这些飞升体积的水液滴中进行了快速（< 1 s）和慢速（> 1 h）化学反应。
This paper describes a fluidic and optical platform for the generation and manipulation of single femtoliter-volume aqueous droplets. Individual droplets were generated on-demand using a microfluidic chamber that confers environmental flow stability. Optical vortex traps were implemented to manipulate and transport the generated droplets, which have a lower refractive index than the immiscible medium in which the droplets are immersed and thus cannot be trapped using conventional optical tweezers. We also demonstrated the ability to shrink and increase the refractive index of the generated droplet, thereby permitting its facile fusion with another droplet using an optical tweezer. To illustrate the versatility of this platform, we have performed both fast (< 1 s) and slow (> 1 h) chemical reactions in these femtoliter-volume aqueous droplets.